Martha Loizeaux: Spectrophotometers and Eggplant Curry, August 28, 2018

NOAA Teacher at Sea

Martha Loizeaux

Aboard NOAA Ship Gordon Gunter

August 22-31, 2018

Mission: Summer Ecosystem Monitoring Survey
Geographic Area of Cruise: Northeast Atlantic Ocean
Date: August 28, 2018

Weather Data from the Bridge

  • Latitude:  39.487 N
  • Longitude:  73.885 W
  • Water Temperature: 25.2◦C
  • Wind Speed:  13.1 knots
  • Wind Direction: WSW
  • Air Temperature: 26.1◦C
  • Atmospheric Pressure:  1017.28 millibars
  • Depth:  30 meters

Science and Technology Log

spectrophotometer
This is the underwater spectrophotometer!

“Underwater spectrophotometer”… say that 10 times fast!  I was lucky enough to steal a few minutes of Audrey Ciochetto’s time while we admired the views from the fly bridge today.  Audrey works with the Colleen Mouw Lab at the University of Rhode Island.  Her lab studies phytoplankton (you may remember that phytoplankton is plankton that is like a plant) and how light from the sun interacts with plankton.  I bet you never thought about that!  It’s amazing stuff!

Audrey and a graduate student from the lab, Kyle Turner, have brought another cool science tool on board, an underwater spectrophotometer.  The ship has pipes hooked up that take water in from 4 meters under the surface of the ocean at a constant flow.  This water goes into the spectrophotometer and the machine gets to work.  It shines light through the water and measures how the light is absorbed (taken in).  Did you know that light travels in waves?  Different colors of light that you see are different wavelengths.  The spectrophotometer can measure 83 different color wavelengths and what happens to them when they shine on the water.

What does happen to light when it shines into the water?  First of all, the water itself absorbs some of the light.  There are also a lot of tiny things in the water that absorb light.  Can you think of some tiny things that might be in the water? You guessed it again!  Phytoplankton is absorbing some of the light, but also other things like tiny particles and dissolved matter will absorb light.  These items will also scatter the light, making it bounce in different directions.  The underwater spectrophotometer measures that too!

filtering Audrey
Audrey filtering water samples to separate particles and plankton

Audrey and Kyle spend some of their day taking samples of the water and filtering out the plankton and particles, leaving only the dissolved matter.  They will also bring some sea water samples back to their lab to separate the phytoplankton from the rest of the particles. By separating all of these factors, scientists can get an idea of how each of these components in the water are responding to light.

The goal of this work is to understand what satellites are seeing.  Scientists rely on satellites out in space to take pictures of what’s happening on Earth.  These satellites can detect the light from the sun shining on Earth.  They can see some color wavelengths as they are absorbed or scattered by different things on our planet.  With the work that Audrey and Kyle are doing, we can better understand the satellite pictures of the ocean and what they mean.  We can understand what’s in the ocean by looking at what the sunlight is doing when it touches the water.  Pretty incredible, right?

The Design of Experiments

Hearing all of these brilliant ideas from Audrey got me thinking about how creative scientists must be to design experiments and investigations to answer questions.
Remember the hypothesis example that Chief Scientist Harvey mentioned in his interview?  It was an idea that scientists came up with after they used monitoring data to discover a pattern of lower populations of herring (fish).

Hypothesis:  “Increasing haddock populations lead to a lower stable state of herring because haddock feed on herring eggs.”

fish stomach contents
Scientists can study the stomach contents of fish to learn what they are eating. Photo courtesy of The Fisherman Magazine.

How would you design an experiment to test this?
Well, the real scientists who did this work examined the stomach contents of haddock to see how much of their diet consisted of herring eggs!  Would you have thought of that?
It was interesting to read about this study in a scientific journal called PNAS (it stands for Proceedings of the National Academy of Sciences), “Role of egg predation by haddock in the decline of an Atlantic herring population.” By Richardson et. al.

Get creative and start thinking of your own ideas to answer questions you have about the world!

 

 

Scientist Spotlight – Tamara Holzwarth-Davis, Physical Science Technician

Tamara is the physical science technician for NOAA National Marine Fisheries Service (NMFS) at Woods Hole.  A technician is someone who is an expert on the equipment and technology used by the scientists.  Today I had a chance to ask Tamara some more questions about her work.

Me – Tell me more about your job.
Tamara – I provide quality control for all of the data brought back by all of the ships involved in our study.  A lot of it is statistical analysis of data [this means looking at data and making sure that it makes sense and is accurate].  I calibrate sensors [make sure they are accurate], process data, and write reports based on the data we find.  We create a yearly atlas of information based on our data that anyone can use to look for trends (such as changes in plankton populations).  I also maintain and coordinate equipment that is needed for the studies.

Me – What part of your job with NOAA did you least expect to be doing?Tamara – I least expected to be so involved with plankton!  I used to do only the hydrography (water chemistry and physical properties) but now I am also involved with plankton data collection.

Tamara on watch
Tamara keeps track of a lot of different things during her watch.

Me – How do you help other people understand and appreciate NOAA’s work?
Tamara – I write the reports and make data available to the public.  People can be reassured that quality control is in place in our monitoring and the data is as accurate as possible.  It is my job to make sure of it!

Me – What do you love about going out to sea?
Tamara – I love the experience of being out at sea and meeting new people!

Personal Log

Our days on the ship are spent collecting data at stations, storytelling and watching the water on the fly bridge, catching up on work, watching sunrises and sunsets.  I’ve been pleasantly surprised by the comfort and commodities (like comfy mattresses and hot showers) and especially, THE FOOD!

food
The food options are outstanding. One night we had king crab legs and tuna steaks.
Margaret chef
Margaret is the best chef EVER.

Here on NOAA Ship Gordon Gunter, we have a wonderful steward staff (cooks and kitchen managers), Margaret and Paul. They always have smiles on their faces when you walk in for meal time and are happy to spread their cheerfulness.  There is always an amazing menu with many items to choose from.  As a vegetarian, I have been blown away by all of the delicious veggie options.  But there is plenty of meat for the carnivores too!  There are always a variety of snacks available as well as healthy options.

Margaret makes homemade cookies and pies, guacamole, crab salad, and eggplant curry, just to name a few.  We all sit down for meals together and share stories.  And there is always dessert!

Did You Know?

Water absorbs red light first.  So, if a fish has red scales when it’s out of the water, under water he will look brown and blend in to his surroundings.  All of the red light will have already been absorbed by the water and there won’t be enough left to reflect off the fish’s scales!

squirrelfish
A squirrelfish can blend in to its surroundings under water. Since it is a red fish, it is hard to see its color since the water has already absorbed the red light from the sun. Photo courtesy of NOAA.

Animals Seen Today

Common dolphins, green sea turtle, brown booby bird, larval hake, larval flounder, larval sea bass, jellyfish

brown booby
Bobby the brown booby stayed with our ship for several hours.
jellyfish jar
A jellyfish we caught in the plankton net!

Martha Loizeaux: Cool Science Tools and Drifter Buoy! August 26, 2018


Susan Dee: Microscopic Sea Life – Days 1-4, May 24, 2018

NOAA Teacher at Sea
Susan Dee
Aboard NOAA Ship Henry B. Bigelow
May 23 – June 7, 2018

Mission:  Spring Ecosystem Monitoring Survey

Geographic Area of Cruise:  Northeastern Coast U.S.
Date: May 24, 2018
Weather Data from Bridge
Latitude: 40°32′
Longitude: 070°45′
Sea Wave Height:  1-2 feet
Wind Speed:  12 knots°
Wind Direction: west
Viability: unrestricted
Air Temperature:  13.5°C
Sky: Few clouds

Science and Technology Log

Tuesday, May 22, I arrived at Newport Naval Base and boarded NOAA Ship Henry B. Bigelow to begin my Teacher at Sea journey by staying overnight on a docked ship.   Day 1 was filled with many new experiences as we headed out to sea.  The Henry B. Bigelow is part of a fleet of vessels commissioned to conduct  fishery surveys. To learn more about the Henry B. Bigelow,  check out this website:  Henry B. Bigelow. The objective of this cruise is to access the hydrographic, planktonic and pelagic components of North East U.S. continental shelf ecosystem.  The majority of the surveys we will take involve  the microbiotic parts of the sea –  phytoplankton, zooplankton and mesoplankton.  Plankton are small microscope organisms in the oceans that are extremely important to the entire Earth ecosystem.  These organisms are the foundation of the entire ocean food web. By studying their populations. scientists can get an accurate picture of the state of  larger ocean organism populations.

Susan and ship
Henry B. Bigelow
Leaving Newport Harbor
Leaving Newport Harbor

Before leaving the dock, I met with Emily Peacock from Woods Hole Oceanographic Institute (WHOI) to learn how to run an Imaging Flow Cytobot instrument that uses video and flow cytometric technology to capture images of phytoplankton. The IFCB was developed by Dr Heidi Sosik and Rob Olsen (WHOI) to get a better understanding of coastal plankton communities. The IFCB runs 24 hours a day collecting sea water and continuously measuring phytoplankton abundance.  Five milliliters of sea water are analyzed every 20 minutes and produces the images shown below.

Imaging Flow CytoBot
Emily Peacock teaching the usage of the Imaging Flow CytoBot (IFCB)

 

Imaging Flow Cytobot IFCB
Imaging Flow Cytobot (IFCB)
phytoplankton
Images of Phytoplankton taken by IFCB

The science party on board is made up of scientists from National Marine Fisheries Service (NMFS) part of NOAA Fisheries Division. The chief scientist, Jerry Prezioso, works out of Narragansett Lab and the lead scientist, Tamara Holzworth Davis, is from the Woods Hole Lab, both from the NOAA Northeast Fisheries Science Center.  Other members of the Science Party are Seabird/Marine Mammal observers and a student  from Maine Maritime Academy.  The Crew and scientist group work together to coordinate sampling stations. The crew gets the ship to the site and aid the scientists in deploying instruments. The scientists collect the data and samples at each station.  The Crew and scientists work together to find the best and most efficient sea route to each  sampling site. Note all the stops for specimen collection on map below. There definitely  has to be a plan!

map of proposed route
Proposed Cruise Track and Survey Locations

 

Personal Log

Because research instrument deployment is done 24 hours a day, the NOAA Corps crew and scientists are divided into two shifts. I am on watch 1200 – 2400 hours, considered the day shift. This schedule is working good for me. I finish duty at midnight, go to sleep till 9:00 AM and rise to be back on duty at noon. Not a bad schedule. Due to clear weather and calm seas, the ship headed east out of Newport Harbor towards the continental shelf and started collecting samples at planned stops.   I joined another group of scientists  observing bird and marine mammal populations from the flying bridge of the ship. Humpback whales and basking sharks breached  several times during the day

It has only been two days but I feel very acclimated to life at sea. I am not seasick, thanks to calm seas and the patch. Finding the way around the ship is getting easier- it is like a maze. Spotting a pod of humpback whales breaching and basking sharks was a highlight of the day. My Biology students back at May River  High School scored great on End of Course Exam. Congratulations May River High School Sharks! Thinking of y’all.

school logo
Love My SHARKS!

Chelsea O’Connell-Barlow: Full Steam Ahead, August 30, 2017

NOAA Teacher at Sea

Chelsea O’Connell-Barlow

Aboard NOAA Ship Bell M. Shimada

August 29 – September 12, 2017

 

Mission: Pacific Hake Survey

Geographic Area of Cruise: NW Pacific Ocean

Date: 8/30/2017

 

Weather Data from the Bridge:

Latitude: 48.472837N

Longitude: -124.676694W

Temperature 59 F

Wind 9.7 knots

Waves 3-5 feet

Science and Technology Log

We have not started fishing yet because we are heading to our first transect off the western coast of the Haida Gwaii archipelago. I thought this would be a perfect time to introduce another research project that is gathering data on the Shimada. One of my roommates, Lynne Scamman, is on-board researching Hazardous Algal Blooms (HABs).

Lynne in Chem lab
Lynne Scamman running wet chemistry tests and identifying phytoplankton.
  1. What are Hazardous Algal Blooms?

They are large numbers of phytoplankton, either diatoms or dinoflagellates, who produce toxins. Phytoplankton are essential to the ecosystem because they produce half of the global oxygen. However under certain circumstances these organisms reproduce rapidly, skyrocketing the population, this is a bloom. Some of these phytoplankton produce toxins. When the populations are low the toxins aren’t a big deal. However, when a bloom of phytoplankton that produce toxins occurs there can be health concerns for organisms exposed to the toxins. We have to consider the marine food chain and something called bioaccumulation. Phytoplankton along with zooplankton create the base of the marine food web. Organisms who eat toxin producing phytoplankton retain the toxin in their body. Then any organism who eats them will also hold that toxin. You can see how the toxin would accumulate along the food chain and potentially hold serious side effects for organisms with high levels of toxin.

  1. Why is research being done on HABs?

HABs are becoming a problem for humans along the coasts and in the Great Lakes. Basically all of the factors that contribute to the increase in HABs are a product of human impact. Global climate change, increased nutrient pollution and global sea trade are all factors contributing to the rise in Hazardous Algal Blooms. We want to monitor so that eventually we will be able to predict when, where and why the HABs will occur.

  1. Why are YOU studying HABs?

One day I walked into my college biology lab and met a guest instructor who specializes in all things phytoplankton related. I was blown away by the complexity that some of these single celled organisms held. The professor shared a few species names and I started investigating. The species that grabbed my attention is called Nematadinium armatum. This organism has a rudimentary eye called a melanosome and nematocysts for hunting, again this is pretty impressive for an organism made of one cell. Once I learned about the variety in this microscopic world and how influential they were to the health of the entire ocean, I knew that I wanted to learn more.

Personal Log

I am still figuratively pinching myself every few hours at just how amazing this experience is to participate in first hand. Yesterday we left the dock of Port Angeles at 10am and the boat hasn’t slowed down since. We did drills to ensure that all aboard knew where to go in case of fire and if we needed to abandon ship. Part of the abandon ship drill is to make sure that everyone has and can get into their Immersion Suit aka “Gumby Suit.” This suit is amazing! This portion of the Pacific is quite cold and the Immersion suit would keep you warm and buoyant until a rescue can occur.

OCB Gumby
Trying on the Immersion suit.

After our drills several of the science crew went up to the Flying Bridge to look for marine mammals. We were cruising between Cape Flattery, Washington and Vancouver Island, British Columbia with high hopes of seeing activity. WOW, we lucked out. We spotted 17 Humpback whales, 2 Harbor porpoise and 2 Dall’s porpoise. We are also seeing several types of sea birds but I am still brushing up with the Sibley to id birds from this area.

Shimada Flags
The Shimada under two flags as it enters Canadian waters.

 

Did You Know?

The island cluster that we are heading to had a name change at the end of 2009. What was formerly called Queen Charlotte Islands is now called Haida Gwaii. This name change came as part of a historic reconciliation between British Columbia and Haida nation. Haida Gwaii translated means “island of the people.”

Haida Gawaii
Map of Haida Gawaii area.

Amanda Dice: Ending Week 1 at Line 8, August 26, 2017

NOAA Teacher at Sea

Amanda Dice

Aboard Oscar Dyson

August 21 – September 2, 2017

 

Mission: Juvenile Pollock Fishery Survey

map cropped
Oscar Dyson moves across the Shelikof Straight to collect the Line 8 samples

Geographic area of cruise: Western Gulf of Alaska

Date: August 26, 2017

Weather Data: 13.2 C, cloudy with light rain

Latitude 57 36.6 N, Longitude 155 .008 N

 

 

Science and Technology Log

As part of this survey, the scientists onboard collect data from what is known as “Line 8”. This is a line of seven sampling stations, positioned only a few miles apart, near the southern opening of Shelikof Straight between Kodiak Island and the Alaskan Peninsula. Water samples are taken at different depths at each sampling station to measure several different properties of the water. This study is focused on profiling water temperature and salinity, and measuring the quantities of nutrients and phytoplankton in the water.

IMG_0988
The CTD rosette is lowered into the water using a winch – as seen from above.

To collect this data, a conductivity and temperature at depth (CTD) instrument is lowered into the water. This instrument can take water samples at different depths, by using its eleven canisters, or Niskin bottles. The water collected in the Niskin bottles will be used to determine the nutrient quantities at each station. The rosette of Niskin bottles also has sensors on it that measure phytoplankton quantities, depth, temperature, and how conductive the water is. Scientists can use the readings from conductivity and temperature meters to determine the salinity of the water.

Each Niskin bottle has a stopper at the top and the bottom. The CTD goes into the water with both ends of each Niskin bottle in the open position. The CTD is then lowered to a determined depth, depending on how deep the water is at each station. There is a depth meter on the CTD that relays its position to computers on board the ship. The survey team communicates its position to the deck crew who operate the winch to raise and lower it.

IMG_1164
Niskin bottles are lowered into the water with the stoppers at both ends open.

When the CTD is raised to the first sampling depth, the survey crew clicks a button on a monitor, which closes the stoppers on both ends of Niskin bottle #1, capturing a water sample inside. The CTD is then raised to the next sampling depth where Niskin bottle #2 is closed. This process continues until all the samples have been collected. A computer on board records the depth, conductivity and temperature of the water as the CTD changes position. A line appears across the graph of this data to show where each sample was taken. After the Niskin bottles on the CTD are filled, it is brought back onto the deck of the ship.

IMG_1173
They let me take control of closing the Niskin bottles at the sampling depths!
CTD screen cropped
I used this screen to read the data coming back from the CTD and to hit the bottle to close each Niskin bottle. The purple horizontal lines on the graph on the right indicate where each one was closed.

Water is collected through a valve near the bottom of each Niskin bottle. A sample of water from each depth is placed in a labeled jar. This study is interested in measuring the quantity of nutrients in the water samples. To do this it is important to have samples without phytoplankton in them. Special syringes with filters are used to screen out any phytoplankton in the samples.

Screen Shot 2017-08-26 at 8.28.56 PM
Syringes with special filters to screen out phytoplankton are used to collect water samples from the Niskin bottles.

The “Line 8” stations have been sampled for nutrient, plankton, and physical water properties for many years. The data from the samples we collected will be added to the larger data set maintained by the Ecosystems and Fisheries-Oceanography Coordinated Investigations (Eco-FOCI), Seattle, Washington. This NOAA Program has data on how the marine ecosystem in this area has changed over the last few decades. When data spans a long time frame, like this study does, scientists can identify trends that might be related to the seasons and to inter-annual variation in ocean conditions. The samples continue to be collected because proper nutrient levels are important to maintaining healthy phytoplankton populations, which are the basis of most marine food webs.

 

IMG_1171
Collecting water samples from a Niskin bottle.

Personal Log

As we travel from one station to the next, I have some time to talk with other members of the science team and the crew. I have really enjoyed learning about places all over the world by listening to people’s stories. Most people aboard this ship travel many times a year for their work or have lived in remote places to conduct their scientific studies. Their stories inspire me to keep exploring the planet and to always search for new things to learn!

Did you know?

Niskin bottles must be lowered into the water with both ends open to avoid getting an air bubble trapped inside of them. Pressure increases as depth under water increases. Niskin bottles are often lowered down below 150 meters, where the pressure can be intense. If an air bubble were to get trapped inside, the pressure at these depths would cause air bubble to expand so much that it might damage the Niskin bottle!