sample sorting – NOAA Teacher at Sea Blog

August 26, 2022August 30, 2022

George Hademenos: A Day in the Life…of a Marine Science Researcher, August 25, 2022

NOAA Teacher at Sea

George Hademenos

Aboard R/V Tommy Munro

July 19 – 27, 2022

Mission: Gulf of Mexico Summer Groundfish Survey

Geographic Area of Cruise: Eastern Gulf of Mexico

Date: August 25, 2022

In this post, I would like to walk you through my interactions and observations with the science research being conducted aboard the R/V Tommy Munro, in particular, the steps that were taken during a trawling process. The entire process involved three stages: Preparing for Sampling, Conducting the Sampling, and Analyzing the Sampling with each stage consisting of six distinct steps.

View the following steps in an interactive tour here: Trawl Sampling Process (Genially)

I. Preparing for Sampling

Step 1: The ship travels to designated coordinates for sampling sites as determined for the particular leg of the Survey by SEAMAP (Southeast Area Monitoring and Assessment Program).

screenshot of a computer screen showing the path that R/V Tommy Munro traveled among sampling sites. The ship's path is a bold blue line connecting sample sites marked in yellow. It's superimposed on an electronic nautical chart. This survey occurred southeast of Florida's Apalachicola Bay and St. George Island. — **Ship Transport to Sampling Site**

Step 2: Once the ship reaches the site, a Secchi disk is attached to a cable and lowered into the water off the side of the ship to determine visibility. When the disk can no longer be seen, the depth is recorded and the disk is raised and secured on ship.

a scientist wearing a life vest stands on a small grated platform that has folded down off the fantail of R/V Tommy Munro. With his left land, he grasps a cable hanging from an A-Frame that extends out of the photo. The cable is attached to a white disk, about the size of an old record, with a weight underneath. — **Deployment of Secchi Disk**

Step 3: A CTD (Conductivity, Temperature, and Depth) unit is then prepared for deployment. It is a rectangular chamber with sensors designed to measure physical properties of the water below including dissolved oxygen, conductivity, transmissivity, and depth.

a conductivity, temperature, and depth probe, mounted inside a rectangular metal cage about 1 foot square and about 3 feet high, sits on deck. a crew member wearing white shrimp boots hooks a cable onto the top of the CTD frame. Another person, mostly out of frame, touches the CTD frame with their right hand, covered in a blue latex glove. — **Preparation of CTD Unit**

Step 4: The CTD unit is powered on and first is submerged just below the surface of the water and left there for three minutes for sensors to calibrate. It is then lowered to a specified depth which is 2 meters above the floor of the body of water to protect the sensors from damage.

the CTD unit, attached to a cable, sinks into dark blue water. — **Deployment of CTD Unit**

Step 5: Once the CTD unit has reached the designated depth, it remains there only for seconds until it is raised up and secured on board the ship.

a science team member, wearing a blue hat, a blue life vest, and blue latext gloves, stands on the deployment platform out the back of R/V Tommy Munro. He grasps the top of the CTD frame as a cable lifts it back out of the water. — **Recovery of CTD Unit**

Step 6: The CTD unit is then turned off and the unit is connected through a cable to a computer in the dry lab for data upload. Once the data upload is completed, the CTD unit is flushed with deionized water using a syringe and plastic tubing and then secured on the side of the ship.

the CTD unit sits on deck, now connected to a computer via a cable to upload the data it collected. — **Data Upload from CTD Unit**

II. Conducting the Sampling

Step 1: The trawling process now begins with the trawl nets thrown off the back of the ship. The nets are connected to two planks, each weighing about 350 lbs, which not only submerges the nets but also provide an angled resistance which keeps the nets open in the form of a cone – optimal for sampling while the ship is in motion.

a view of the fantail of R/V Tommy Munro, from an upper deck. we are looking through the rigging of the trawl frames. two large planks rest on the lower deck, connected to ropes and lines. the trawl net, connected to the planks, extends out the back of the fantail. It is just visible below the surface, a turquoise-colored cone submerged in a blue sea. — **Preparation of the Trawling Process Part 1**

another view of the fantail of R/V Tommy Munro from an upper deck, through extensive rigging and frames. the trawl net is further extended; now the large planks are lowering off the back deck as well, suspended by lines connected to a pulley in an A-frame. it is a clear day and the water is very smooth. — **Preparation of the Trawling Proces**s Part 2

Step 2: Once the trawl nets have been released into the water from the ship, the ship starts up and continues on its path for 30 minutes as the nets are trapping marine life it encounters.

a view of the fantail of R/V Tommy Munro from an upper deck. the trawl net is fully deployed and no longer visible. a crew member sweeps the deck. — **Onset of the Trawling Process**

Step 3: After 30 minutes has transpired, a siren sounds and the ship comes to a stop. The two weighted planks are pulled upon the ship followed by the trawl nets.

a view of the A-frame at the fantail R/V Tommy Munro as the trawl net rises from the ocean. The two spreader panels are suspended from separate lines running through the central pulley. behind those, the top of the trawl net is visible above the water. a crew member guides the spreader doors with his left hand, holding the lines with his right hand. — **Conclusion of the Trawling Process** Part 1

the spreader doors are now resting on the fantail deck again. two crewmembers, wearing life jackets, pull the trawl net back on board. — **Conclusion of the Trawling Process** Part 2

Step 4: The trawl nets are raised and hoisted above buckets for all specimens to be collected. Then begins the process of separation. In the first separation, the marine life is separated from seaweed, kelp and other debris. The buckets with marine life and debris are then weighed and recorded.

a crewmember (only partially visible) empties the contents of the trawl net into a blue plastic basket. it looks like it's mostly sargassum. — **Content Collection from the Trawl** Part 1

four plastic baskets on deck hold the sorted contents of the trawl. one has larger fish; another contains only a single fish; a third is a jumble of seaweed and sargassum, and may represent the remainder to sort; the contents of the fourth are not visible. a crewmember wearing a life vest and gloves leans over the baskets. another crewmember, only partially visible, looks on. — **Content Collection from the Trawl** Part 2

Step 5: The bucket(s) with marine life are emptied upon a large table on the ship’s stern for separation according to species.

a pile of fish on a large metal sorting table. we can see snappers, a trigger fish, and many lionfish. a stack of white sorting baskets rests adjacent to the pile. — **Separation Based on Species** Part 1

a gloved hand reaches toward the pile of fish on the metal sorting table. (this photo was taken from the same vantage point as the previous one.) — **Separation Based on Species** Part 2

Step 6: Each species of marine life is placed in their own tray for identification, examination, and measurements inside the wet lab.

two gloved crewmembers sort fish into smaller white baskets on a large metal sorting table. the table is on the back deck of the ship, and we can see smooth ocean conditions in the background. the crewmember in the foreground considers a small fish he has picked up from the remaining unsorted pile. the other crewmember looks on. — **Species Sorted in Trays** Part 1

a close-up view of the sorting basket containing only lionfish. — **Species Sorted in Trays** Part 2

III. Analyzing the Sampling

Step 1: After all species were grouped in their trays, all trays were taken into the wet lab for analysis. Each species was positively identified, counted, and recorded.

a direct view of three fish of different species, lined up on the metal sorting table. the third is a spotfin butterflyfish. — **Tray Transport to Wet Lab**

Step 2: Once each species was identified and counted, the total number of species was weighed while in the tray (accounting for the mass of the tray) and recorded on a spreadsheet to a connected computer display system.

a view of a scale. — **Total Weight Measurements**

Step 3: For each species, the length of each specimen was recorded using a magnetic wand with a sensor that facilitated the electronic recording of the value into a spreadsheet.

two hands, wearing latex gloves, measure a small lionfish on the electronic measuring board. the scientist holds the fish against the board with his left hand and with his right hand marks the length with the magnetic stylus. — **Individual Length Measurements**

Step 4: Weights of the collected species were recorded for the first sample and every fifth one that followed.

the gloved arm places the small lionfish on the scale behind the fish measuring board. — **Individual Weight Measurements**

Step 5: If time permitted between samplings, the sex of selected specimens for a species was determined and recorded.

gloved hands cut into a small lionfish to remove the fish's gonads. — **Individual Species Sex Identification**

Step 6:Once the entire sampling was analyzed, selected samples of specimens were placed in a baggie and stored in a freezer for further analysis with the remaining specimens returned to a larger bucket and thrown overboard into the waters. The separation table was cleaned with a hose and buckets were piled in preparation for the next sampling.

view out the fantail of R/V Tommy Munro from the lower deck. the trawl net and spreader doors lay on the deck, not currently in use. the sun shines on calm seas. — **Finalize Process and Prepare for Next**

In this installment of my exercise of the Ocean Literacy Framework, I would like to ask you

to respond to three questions about the fifth essential principle (The ocean supports a great diversity of life and ecosystems.), presented in a Padlet accessed by the following link:

https://tinyurl.com/427xp9p3

Remember, there are no right or wrong answers – the questions serve not as an opportunity to answer yes or no, or to get answers right or wrong; rather, these questions serve as an opportunity not only to assess what you know or think about the scope of the principle but also to learn, explore, and investigate the demonstrated principle. If you have any questions or would like to discuss further, please indicate so in the blog and I would be glad to answer your questions and initiate a discussion.

July 21, 2015August 21, 2021

Michael Wing: How to Sample the Sea, July 20, 2015

NOAA Teacher at Sea
Michael Wing
Aboard R/V Fulmar
July 17 – 25, 2015

Mission: 2015 July ACCESS Cruise
Geographical Area of Cruise: Pacific Ocean west of Marin County, California
Date: July 20, 2015

Weather Data from the Bridge: 15 knot winds gusting to 20 knots, wind waves 3-5’ and a northwest swell 3-4’ four seconds apart.

Science and Technology Log

On the even-numbered “lines” we don’t just survey birds and mammals. We do a lot of sampling of the water and plankton.

Wing on Fulmar — Wing at rail of the R/V Fulmar

We use a CTD (Conductivity – Temperature – Depth profiler) at every place we stop. We hook it to a cable, turn it on, and lower to down until it comes within 5-10 meters of the bottom. When we pull it back up, it has a continuous and digital record of water conductivity (a proxy for salinity, since salty water conducts electricity better), temperature, dissolved oxygen, fluorescence (a proxy for chlorophyll, basically phytoplankton), all as a function of depth.

We also have a Niskin bottle attached to the CTD cable. This is a sturdy plastic tube with stoppers at both ends. The tube is lowered into the water with both ends cocked open. When it is at the depth you want, you clip a “messenger” to the cable. The messenger is basically a heavy metal bead. You let go, it slides down the cable, and when it strikes a trigger on the Niskin bottle the stoppers on both ends snap shut. You can feel a slight twitch on the ship’s cable when this happens. You pull it back up and decant the seawater that was trapped at that depth into sample bottles to measure nitrate, phosphate, alkalinity, and other chemical parameters back in the lab.

When we want surface water, we just use a bucket on a rope of course.

We use a hoop net to collect krill and other zooplankton. We tow it behind the boat at a depth of about 50 meters, haul it back in, and wash the contents into a sieve, then put them in sample bottles with a little preservative for later study. We also have a couple of smaller plankton nets for special projects, like the University of California at Davis graduate student Kate Davis’s project on ocean acidification, and the plankton samples we send to the California Department of Health. They are checking for red tides.

We use a Tucker Trawl once a day on even numbered lines. This is a heavy and complicated rig that has three plankton nets, each towed at a different depth. It takes about an hour to deploy and retrieve this one; that’s why we don’t use it each time we stop. The Tucker trawl is to catch krill; which are like very small shrimp. During the day they are down deep; they come up at night.

A mass of krill we collected. The black dots are their eyes.

What happens to these samples? The plankton from the hoop net gets sent to a lab where a subsample is taken and each species in the subsample is counted very precisely. The CTD casts are shared by all the groups here – NOAA, Point Blue Conservation Science, the University of California at Davis, San Francisco State University. The state health department gets its sample. San Francisco State student Ryan Hartnett has some water samples he will analyze for nitrate, phosphate and silicate. All the data, including the bird and mammal sightings, goes into a big database that’s been kept since 2004. That’s how we know what’s going on in the California Current. When things change, we’ll recognize the changes.

Personal Log

They told me “wear waterproof pants and rubber boots on the back deck, you’ll get wet.” I thought, how wet could it be? Now I understand. It’s not that some water drips on you when you lift a net up over the stern of the boat – although it does. It’s not that waves splash you, although that happens too. It’s that you use a salt water hose to help wash all of the plankton from the net into a sieve, and then into a container, and to fill wash bottles and to wash off the net, sieve, basins, funnel, etc. before you arrive at the next station and do it all again. It takes time, because you have to wash ALL of the plankton from the end of the net into the bottle, not just some of it. You spend a lot of time hosing things down. It’s like working at a car wash except with salty water and the deck is pitching like a continuous earthquake.

The weather has gone back to “normal”, which today means 15 knot winds gusting to 20 knots, wind waves 3-5’ and a northwest swell 3-4’ only four seconds apart. Do the math, and you’ll see that occasionally a wind wave adds to a swell and you get slapped by something eight feet high. We were going to go to Bodega Bay today; we had to return to Sausalito instead because it’s downwind.

The sea state today. Some waves were pretty big.

We saw a lot of humpback whales breaching again and again, and slapping the water with their tails. No, we don’t know why they do it although it just looks like fun. No, I didn’t get pictures. They do it too fast.

Did You Know? No biologist or birder uses the word “seagull.” They are “gulls”, and there are a lot of different species such as Western gulls, California gulls, Sabine’s gulls and others. Yes, it is possible to tell them apart.

May 4, 2015August 21, 2021

Emily Whalen: Station 381–Cashes Ledge, May 1, 2015

NOAA Teacher at Sea
Emily Whalen
Aboard NOAA Ship Henry B. Bigelow
April 27 – May 10, 2015

Mission: Spring Bottom Trawl Survey, Leg IV
Geographical Area of Cruise: Gulf of Maine
Date: May 1, 2015

Weather Data from the Bridge:
Winds: Light and variable
Seas: 1-2ft
Air Temperature: 6.2^○C
Water Temperature: 5.8^○C

Science and Technology Log:

Earlier today I had planned to write about all of the safety features on board the Bigelow and explain how safe they make me feel while I am on board. However, that was before our first sampling station turned out to be a monster haul! For most stations I have done so far, it takes about an hour from the time that the net comes back on board to the time that we are cleaning up the wetlab. At station 381, it took us one minute shy of three hours! So explaining the EEBD and the EPIRB will have to wait so that I can describe the awesome sampling we did at station 381, Cashes Ledge.

This is a screen that shows the boats track around the Gulf of Maine. The colored lines represent the sea floor as determined by the Olex multibeam. This information will be stored year after year until we have a complete picture of the sea floor in this area!

Before I get to describing the actual catch, I want to give you an idea of all of the work that has to be done in the acoustics lab and on the bridge long before the net even gets into the water.

The bridge is the highest enclosed deck on the boat, and it is where the officers work to navigate the ship. To this end, it is full of nautical charts, screens that give information about the ship’s location and speed, the engine, generators, other ships, radios for communication, weather data and other technical equipment. After arriving at the latitude and longitude of each sampling station, the officer’s attention turns to the screen that displays information from the Olex Realtime Bathymetry Program, which collects data using a ME70 multibeam sonar device attached to bottom of the hull of the ship .

Traditionally, one of the biggest challenges in trawling has been getting the net caught on the bottom of the ocean. This is often called getting ‘hung’ and it can happen when the net snags on a big rock, sunken debris, or anything else resting on the sea floor. The consequences can range from losing a few minutes time working the net free, to tearing or even losing the net. The Olex data is extremely useful because it can essentially paint a picture of the sea floor to ensure that the net doesn’t encounter any obstacles. Upon arrival at a site, the boat will cruise looking for a clear path that is about a mile long and 300 yards wide. Only after finding a suitable spot will the net go into the water.

Check out this view of the seafloor. On the upper half of the screen, there is a dark blue channel that goes between two brightly colored ridges. That's where we dragged the net and caught all of the fish! — Check out this view of the seafloor. On the upper half of the screen, there is a dark blue channel that goes between two brightly colored ridges. We trawled right between the ridges and caught a lot of really big fish!

The ME70 Multibeam uses sound waves to determine the depth of the ocean at specific points. It is similar to a simpler, single stream sonar in that it shoots a wave of sound down to the seafloor, waits for it to bounce back up to the ship and then calculates the distance the wave traveled based on the time and the speed of sound through the water, which depends on temperature. The advantage to using the multibeam is that it shoots out 200 beams of sound at once instead of just one. This means that with each ‘ping’, or burst of sound energy, we know the depth at many points under the ship instead of just one. Considering that the multibeam pings at a rate of 2 Hertz to 0.5 Herts, which is once every 0.5 seconds to 2 seconds, that’s a lot of information about the sea floor contour!

This is what the nautical chart for Cashes Ledge looks like. The numbers represent depth in fathoms. The light blue lines are contour lines. The places where they are close together represent steep cliffs. The red line represents the Bigelow’s track. You can see where we trawled as a short jag between the L and the E in the word Ledge

The stations that we sample are randomly selected by a computer program that was written by one of the scientists in the Northeast Fisheries Science Center, who happens to be on board this trip. Just by chance, station number 381 was on Cashes Ledge, which is an underwater geographical feature that includes jagged cliffs and underwater mountains. The area has been fished very little because all of the bottom features present many hazards for trawl nets. In fact, it is currently a protected area, which means the commercial fishing isn’t allowed there. As a research vessel, we have permission to sample there because we are working to collect data that will provide useful information for stock assessments.

My watch came on duty at noon, at which time the Bigelow was scouting out the bottom and looking for a spot to sample within 1 nautical mile of the latitude and longitude of station 381. Shortly before 1pm, the CTD dropped and then the net went in the water. By 1:30, the net was coming back on board the ship, and there was a buzz going around about how big the catch was predicted to be. As it turns out, the catch was huge! Once on board, the net empties into the checker, which is usually plenty big enough to hold everything. This time though, it was overflowing with big, beautiful cod, pollock and haddock. You can see that one of the deck crew is using a shovel to fill the orange baskets with fish so that they can be taken into the lab and sorted!

You can see the crew working to handling all of the fish we caught at Cashes Ledge. How many different kinds of fish can you see? Photo by fellow volunteer Joe Warren

At this point, I was standing at the conveyor belt, grabbing slippery fish as quickly as I could and sorting them into baskets. Big haddock, little haddock, big cod, little cod, pollock, pollock, pollock. As fast as I could sort, the fish kept coming! Every basket in the lab was full and everyone was working at top speed to process fish so that we could empty the baskets and fill them up with more fish! One of the things that was interesting to notice was the variation within each species. When you see pictures of fish, or just a few fish at a time, they don’t look that different. But looking at so many all at once, I really saw how some have brighter colors, or fatter bodies or bigger spots. But only for a moment, because the fish just kept coming and coming and coming!

Finally, the fish were sorted and I headed to my station, where TK, the cutter that I have been working with, had already started processing some of the huge pollock that we had caught. I helped him maneuver them up onto the lengthing board so that he could measure them and take samples, and we fell into a fish-measuring groove that lasted for two hours. Grab a fish, take the length, print a label and put it on an envelope, slip the otolith into the envelope, examine the stomach contents, repeat.

Cod, pollock and haddock in baskets waiting to get counted and measured. Photo by Watch Chief Adam Poquette.

Some of you have asked about the fish that we have seen and so here is a list of the species that we saw at just this one site:

Pollock
Haddock
Atlantic wolffish
Cod
Goosefish
Herring
Mackerel
Alewife
Acadian redfish
Alligator fish
White hake
Red hake
American plaice
Little skate
American lobster
Sea raven
Thorny skate
Red deepsea crab

Goosefish. Does this remind you of anyone you know?

Mackerel. Possibly the best looking fish in the sea.

I think it’s human nature to try to draw conclusions about what we see and do. If all we knew about the state of our fish populations was based on the data from this one catch, then we might conclude that there are tons of healthy fish stocks in the sea. However, I know that this is just one small data point in a literal sea of data points and it cannot be considered independently of the others. Just because this is data that I was able to see, touch and smell doesn’t give it any more validity than other data that I can only see as a point on a map or numbers on a screen. Eventually, every measurement and sample will be compiled into reports, and it’s that big picture over a long period of time that will really allow give us a better understanding of the state of affairs in the ocean.

Sunset from the deck of the Henry B. Bigelow

Personal Log

Lunges are a bit more challenging on the rocking deck of a ship!

It seems like time is passing faster and faster on board the Bigelow. I have been getting up each morning and doing a Hero’s Journey workout up on the flying bridge. One of my shipmates let me borrow a book that is about all of the people who have died trying to climb Mount Washington. Today I did laundry, and to quote Olaf, putting on my warm and clean sweatshirt fresh out of the dryer was like a warm hug! I am getting to know the crew and learning how they all ended up here, working on a NOAA ship. It’s tough to believe but a week from today, I will be wrapping up and getting ready to go back to school!

April 30, 2015August 21, 2021

Emily Whalen: Trawling in Cape Cod Bay, April 29, 2015

NOAA Teacher at Sea
Emily Whalen
Aboard NOAA Ship Henry B. Bigelow
April 27 – May 10, 2015

Mission: Spring Bottom Trawl Survey, Leg IV
Geographical Area of Cruise: Gulf of Maine
Date: April 29, 2015

Weather Data:
GPS location: 42^○51.770’N, 070^○43.695’W
Sky condition: Cloudy
Wind: 10 kts NNW
Wave height: 1-2 feet
Water temperature: 6.2^○C
Air temperature: 8.1^○C

Science and Technology Log:

On board the Henry B. Bigelow we are working to complete the fourth and final leg of the spring bottom trawl survey. Since 1948, NOAA has sent ships along the east coast from Cape Hatteras to the Scotian Shelf to catch, identify, measure and collect the fish and invertebrates from the sea floor. Scientists and fishermen use this data to assess the health of the ocean and make management decisions about fish stocks.

What do you recognize on this chart? Do you know where Derry, NH is on the map? — This is the area that we will be trawling. Each blue circle represents one of the sites that we will sample. We are covering a LOT of ground! Image courtesy of NOAA.

Today I am going to give you a rundown of the small role that I play in this process. I am on the noon to midnight watch with a crew of six other scientists, which means that we are responsible for processing everything caught in the giant trawl net on board during those hours. During the first three legs of the survey, the Bigelow has sampled over 300 sites. We are working to finish the survey by completing the remaining sites, which are scattered throughout Cape Cod Bay and the Gulf of Maine. The data collected on this trip will be added to data from similar trips that NOAA has taken each spring for almost 60 years. These huge sets of data allow scientists to track species that are dwindling, recovering, thriving or shifting habitats.

At each sampling station, the ship first drops a man-sized piece of equipment called a CTD to the sea floor. The CTD measures conductivity, temperature and depth, hence its name. Using the conductivity measurement, the CTD software also calculates salinity, which is the amount of dissolved salt in the water. It also has light sensors that are used to measure how much light is penetrating through the water.

While the CTD is in the water, the deck crew prepares the trawl net and streams it from the back of the ship. The net is towed by a set of hydraulic winches that are controlled by a sophisticated autotrawl system. The system senses the tension on each trawl warp and will pay out or reel in cable to ensure that the net is fishing properly.

Once deployed, the net sinks to the bottom and the ship tows it for twenty minutes, which is a little more than one nautical mile. The mouth of the net is rectangular so that it can open up wide and catch the most fish. The bottom edge of the mouth has something called a rockhopper sweep on it, which is made of a series of heavy disks that roll along the rocky bottom instead of getting hung up or tangled. The top edge of the net has floats along it to hold it wide open. There are sensors positioned throughout the net that send data back to the ship about the shape of the net’s mouth, the water temperature on the bottom, the amount of contact with the bottom, the speed of water through the net and the direction that the water is flowing through the net. It is important that each tow is standardized like this so that the fish populations in the sample areas aren’t misrepresented by the catch. For example, if the net was twisted or didn’t open properly, the catch might be very small, even in an area that is teaming with fish.

Do you think this is what trawl nets looked like in 1948? — This is what the net looks like when it is coming back on board. The deck hands are guiding the trawl warps onto the big black spools. The whole process is powered by two hydraulic winches.

After twenty minutes, the net is hauled back onto the boat using heavy-duty winches. The science crew changes into brightly colored foul weather gear and heads to the wet lab, where we wait to see what we’ve caught in the net. The watch chief turns the music up and everyone goes to their station along a conveyor belt the transports the fish from outside on the deck to inside the lab. We sort the catch by species into baskets and buckets, working at a slow, comfortable pace when the catch is small, or at a rapid fire, breakneck speed when the catch is large.

If you guessed 'sponges', then you are correct! — This is the conveyor belt that transports the catch from the deck into the wetlab. The crew works to sort things into buckets. Do you know what these chunky yellow blobs that we caught this time are?

After that, the species and weight of each container is recorded into the Fisheries Scientific Computing System (FSCS), which is an amazing software system that allows our team of seven people to collect an enormous amount of data very quickly. Then we work in teams of two to process each fish at work stations using a barcode scanner, magnetic lengthing board, digital scale, fillet knives, tweezers, two touch screen monitors, a freshwater hose, scannable stickers, envelopes, baggies, jars and finally a conveyor belt that leads to a chute that returns the catch back to the ocean. To picture what this looks like, imagine a grocery store checkout line crossed with an arcade crossed with a water park crossed with an operating room. Add in some music playing from an ipod and it’s a pretty raucous scene!

The data that we collect for each fish varies. At a bare minimum, we will measure the length of the fish, which is electronically transmitted into FSCS. For some fish, we also record the weight, sex and stage of maturity. This also often includes taking tissue samples and packaging them up so that they can be studied back at the lab. Fortunately, for each fish, the FSCS screen automatically prompts us about which measurements need to be taken and samples need to be kept. For some fish, we cut out and label a small piece of gonad or some scales. We collect the otoliths, or ear bones from many fish.

It does not look this neat and tidy when we are working! — These are the work stations in the wet lab. The cutters stand on the left processing the fish, and the recorders stand on the right.These bones can be used to determine the age of each fish because they are made of rings of calcium carbonate that accumulate over time.

Most of the samples will got back to the Northeast Fisheries Science Center where they will be processed by NOAA scientists. Some of them will go to other scientists from universities and other labs who have requested special sampling from the Bigelow. It’s like we are working on a dozen different research projects all at once!

Something to Think About:

Below are two pictures that I took from the flying bridge as we departed from the Coast Guard Station in Boston. They were taken just moments apart from each other. Why do you think that the area in the first picture has been built up with beautiful skyscrapers while the area in the second picture is filled with shipping containers and industry? Which area do you think is more important to the city? Post your thoughts in the comment section below.

Rows of shipping containers. What do you think is inside them?

Downtown Boston. Just a mile from the shipping containers. Why do you think this area is so different from the previous picture?

Personal Log

Believe it or not, I actually feel very relaxed on board the Bigelow! The food is excellent, my stateroom is comfortable and all I have to do is follow the instructions of the crew and the FSCS. The internet is fast enough to occasionally check my email, but not fast enough to stream music or obsessively read articles I find on Twitter. The gentle rocking of the boat is relaxing, and there is a constant supply of coffee and yogurt. I have already read one whole book (Paper Towns by John Greene) and later tonight I will go to the onboard library and choose another. That said, I do miss my family and my dog and I’m sure that in a few days I will start to miss my students too!

If the description above doesn’t make you want to consider volunteering on a NOAA cruise, maybe the radical outfits will. On the left, you can see me trying on my Mustang Suit, which is designed to keep me safe in the unlikely event that the ship sinks. On the right, you can see me in my stylish yellow foul weather pants. They look even better when they are covered in sparkling fish scales!

Seriously, they keep me totally dry! — Banana Yellow Pants: SO 2015! Photo taken by fellow volunteer Megan Plourde.

Seriously, do I look awesome, or what? — This is a Mustang Suit. If you owned one of these, where would you most like to wear it? Photo taken by IT Specialist Heidi Marotta.

That’s it for now! What topics would you like to hear more about? If you post your questions in the comment section below, I will try to answer them in my next blog post.

August 8, 2014August 21, 2021

Kacey Shaffer: Fish Scales. Fish Tales. August 8, 2014

NOAA Teacher at Sea

Kacey Shaffer

Aboard NOAA Ship Oscar Dyson

July 26 – August 13, 2014

Mission: Walleye Pollock Survey

Geographical Location: Bering Sea

Date: August 8, 2014
Weather information from the Bridge:

Air Temperature: 11° C

Wind Speed: 27 knots

Wind Direction: 30°

Weather Conditions: High winds and high seas

Latitude: 60° 35.97 N

Longitude: 178° 56.08 W
Science and Technology Log:

If you recall from my last post we left off with fish on the table ready to be sorted and processed. Before we go into the Wet Lab/Fish Lab we need to get geared up. Go ahead and put on your boots, bibs, gloves and a jacket if you’re cold. You should look like this when you’re ready for work…

This is the gear you'll need in the Wet Lab. It can get pretty slimy in there! (Photo Credit: Emily) — This is the gear you’ll need in the Wet Lab. It can get pretty slimy in there! (Photo Credit: Emily)

The first order of business is sorting the catch. We don’t have a magic net that only catches Pollock. Sometimes we pick up other treats along the way. Some of the cool things we’ve brought in are crabs, squid, many types of jellyfish and the occasional salmon. One person stands on each side of the conveyor belt and picks these other species out so they aren’t weighed in with our Pollock catch. It is very important that we only weigh Pollock as we sort so our data are valid. After all the Pollock have been weighed, we then weigh the other items from the haul. Here are some shots from the conveyor belt.

Kacey lifts the door on the table so the fish will slide down onto the conveyor belt. This is when other species are pulled out. (Photo Credit: Sandi)

At the end of the conveyor belt, Pollock are put into baskets, weighed and put into the sorting bin. (Photo Credit: Sandi)

Not every single fish in our net is put into the sorting bin. Only random selection from the catch goes to the sorting bin. The remaining fish from the haul are returned back to the sea. Those fish who find themselves in the sorting bin are cut open to determine their sex. You can’t tell the sex of the fish just by looking at the outside. You have to cut them open, slide the liver to the side and look for the reproductive organs. Males have a rope-like strand as testes. Females have ovaries, which are sacs similar to the stomach but are a distinctly different color.

This is the sorting bin. Can you guess what Blokes and Sheilas means?

The white, rope-like structure is the male reproductive organ.

The pinkish colored sac is one of the female's ovaries. It contains thousands of eggs! — The pinkish colored sac is one of the female’s ovaries. It contains thousands of eggs!

Kacey uses a scalpel to cut the fish. She slides the liver out and looks for the reproductive organs. Is it a male or female? (Photo Credit: Darin)

Okay, no more slicing open fish. For now! The next step is to measure the length of all the fish we just separated by sex. One of the scientists goes to the blokes side and another goes to the sheilas side. We have a handy-dandy tool used to measure and record the lengths called an Ichthystick. I can’t imagine processing fish without it!

The Ichthystick is used to record the length of fish. A special tool held in the hand has a magnet inside that makes a connection with a magnet strip inside the board. It automatically registers a length and records it in a computer program called Clams

Kacey measures the length of a male with the Ichthystick. She holds the tool in her right hand and places it at the fork in the fish’s tail. A special sound alerts her when the data is recorded. (Photo Credit: Darin)

That is the end of the line for those Pollock but we still have a basket waiting for us. A random sample is pulled off the conveyor belt and set to the side for another type of data collection. The Pollock in this special basket will be individually weighed, lengths will be taken and a scientist will determine if it is a male or female. Then we remove the otoliths. What are otoliths? They are small bones inside a fish’s skull that can tell us the age of the fish. Think of a tree and how we can count the rings of a tree to know how old it is. This is the same concept. For this special sample we remove the otoliths, which are labeled and given to a lab on land where a scientist will carefully examine them under a microscope. The scientist will be able to connect the vial containing the otoliths to the other data collected on that fish (length, weight, sex) because each fish in this sample is given a unique specimen number. This is all part of our mission, which is analyzing the health and population of Pollock in the Bering Sea!

Kacey scans a barcode placed on an otolith vial. Robert is removing the otoliths from each fish and Kacey places them in the vial. It is important to make sure the otoliths are placed in the vial that corresponds to the fish Robert measured. (Photo Credit: Emily)

Kacey removes an otolith from a fish Robert cut open. The otoliths are placed in the vial Kacey is holding. (Photo Credit: Emily)

At this point we have just about collected all the data we need for this haul. Each time we haul in a catch this process is completed. As of today, our survey has completed 28 hauls. Thank goodness we have a day shift and a night shift to share the responsibility. That would be a lot of fish for one crew to process! For our next topic we’ll take a look at how the data is recorded and what happens after we’ve completed our mission. By the way, “blokes” are males and “sheilas” are females. Now please excuse us while we go wash fish scales off of every surface in the Wet Lab, including ourselves!

Personal Log:

Just so you know, we’re not starving out here. In fact, we’re stuffed to the gills – pun completely intended. Our Chief Steward Ava and her assistant Adam whip up some delicious meals. Since I am on night shift I do miss the traditional breakfast served each morning. Sometimes, like today, I am up for lunch. I’m really glad I was or I would have missed out on enchiladas. That would have been a terrible crisis! Most people who know me realize there is never enough Mexican food in my life! Tacos (hard and soft), rice and beans were served along with the enchiladas. Each meal is quite a spread! If I have missed lunch I’ll grab a bowl of cereal to hold me over until supper. I bet you’ll never guess we eat a lot of seafood on board. There is usually a fish dish at supper. We even had crab legs one night and fried shrimp another. Some other supper dishes include pork chops, BBQ ribs, baked steak, turkey, rice, mashed potatoes, and macaroni and cheese plus there are always a couple vegetable dishes to choose from. We can’t forget about dessert, either. Cookies, cakes, brownies or pies are served at nearly every meal. It didn’t take long for me to find the ice cream cooler, either. What else would one eat at midnight?!

Ava and Adam are always open to suggestions as well. Someone told Ava the night shift Science Crew was really missing breakfast foods so a few days ago we had breakfast for supper. Not only did they make a traditional supper meal, they made a complete breakfast meal, too! We had pancakes, waffles, bacon, eggs, and hashbrowns. It was so thoughtful of them to do that for us, especially on top of making a full meal for the rest of the crew. Thanks Ava and Adam!

There are situations where a crew member might not be able to make it to the Mess during our set serving schedule. Deck Crew could be putting a net in or taking it out or Science Crew could be processing a catch. We never have to worry, though. Another great thing about Ava and Adam is they will make you a plate, wrap it up and put it in the fridge so you have a meal for later.

Like I said, we’re not going hungry any time soon! Here are some shots from the Mess Deck (dining room).

Mess Deck on the Oscar Dyson. Can you guess why there are tennis balls on the legs of the chairs?

There are always multiple options for every meal. If you’re hungry on this ship you must be the pickiest eater on Earth!

Did you know?

Not only are otoliths useful to scientists during stock assessment, they help the fish with balance, movement and hearing.

July 7, 2014August 21, 2021

Crystal Davis, When Science Goes Wrong, July 6 2014

NOAA Teacher at Sea

Crystal Davis

Aboard NOAA Ship Oregon II

June 23 – July 7, 2014

Mission: SEAMAP Groundfish Survey

Geographical area of cruise: Gulf of Mexico

Date: Sunday July 6, 2014

Weather: Clear and Sunny

Waves: 1-2 feet

Science and Technology Log:

The title of this post should actually be, “when science doesn’t go exactly as planned,” but that doesn’t sound quite as dramatic.

If you have ever written a lab report, you know that there is a section for procedures (what you did). The procedures need to be explicit so that they can be replicated by another individual who will obtain the same results. If your experiment cannot be replicated, your experiment is not valid and is useless. While it is okay for your hypothesis to be different than your expected outcomes, you always have to follow your procedure.

But . . . what if you’re in the middle of the ocean potentially hundreds of miles away from shore and on a deadline? You can’t go back to shore. There are at least thirty people on your boat and a lot of money invested in this data collection. Yet you still have to come up with a way to complete your survey. The events that follow are incidents that occurred on the Oregon II from July 26-July 6 and how the scientists coped with these situations.

Sharks

Juvenile Hammerhead Shark — Hammerhead Shark, Courtesy of Robin Gropp

In August, NOAA conducts a Longline Survey surveying sharks. Sharks are captured, identified and many are tagged with tracking devices to monitor the location and population density of sharks. Other sharks are sampled to determine age, analyze growth, sexual maturity and study stomach contents.

When sharks are captured in the trawl net on the Groundfish Survey, Robin (the intern) has been releasing them back into the Gulf after collecting data. However, not all of the sharks survive being pulled up in the net. The picture to the left is of a juvenile Hammerhead that did not survive. While this saddens me, he has been frozen and will be used to educate students in the outreach programs that NOAA participates in.

Nature vs Science

Waves crashing on the bow of the Oregon II. This picture was not taken on my survey, but this is what the weather felt like to me.

Sometimes mother nature interferes with the survey and things don’t go exactly as planned. For the first week of my trip we ran into some bad weather. There was a series of storms that came off the coast bringing rain, thunder, lightning and waves that were five to seven feet high. The weather conditions were so bad that the day shift couldn’t immediately collect data at a number of stations. They spent a lot of time waiting for the squalls to pass until it was safe to collect data. In fact, the weather in the Fall Groundfish Survey is so bad that there are a few extra days built in to run from hurricanes.

This morning we were trawling off the mouth of the Mississippi River and brought up a net full of sargassum (seaweed). The entire net, all 42 feet of it, was completely full of sargassum and very little marine life. No one on the boat had seen this much sargassum in the net before. This catch had to be thrown back overboard because the data is not usable. Basically, with that much sargassum in the net, the scientists are not sure if the trawl was fished properly. There is the possibility that because the net was so heavy, it was bogged down, uneven or not scraping the bottom of the ocean floor evenly.

The beginning of a squall courtesy of Andre DeBose

Squall moving the doors on the trawl net courtesy of Andre DeBose

Trawl Net filled with Sargassum

Warren and Mike checking to see if we are keeping the trawl

Mike and Eloy checking out the Sargassum

Formalin

On the Oregon II, plankton samples are preserved in Formalin (40% Formaldehyde). Formalin is a clear substance that stops cells from breaking down. A few days ago we noticed that the Formalin was no longer clear, it was in fact opaque. You can see this in the picture on the left. My night shift crew was worried that it was no longer useful and that we could not bring planktons samples back to the lab in Pascagoula. However, our chief scientist assured us that we could still use the Formalin and that it would be effective. The color change indicated that the base in the mixture was breaking down but since we only have a couple more days of plankton sampling, that it will be fine.

Personal Log:

I arrived back home last night and let me tell you it is strange to be back on land. I was never seasick on the Oregon II, but I am 100% landsick now. I find myself swaying from side to side anytime I’m standing still (Dock Rock is the official term). And when I woke up last night to get a glass of water, I fell over because I was swaying so much. It’s actually pretty funny but I will be glad once this goes away.

I’m still taking in my experience from the last two weeks but I am so grateful for the people I met and was able to work with. Everyone on the Oregon II was helpful, accommodating, friendly and made me feel at home. They took time out of their day to answer my questions, give me tours, tell me stories about their history and adventures on board, go over their research and they were genuinely interested in what I do in my classroom. XO (Executive Officer) LCDR Eric Johnson spent a good chunk of his time telling me about the NOAA Corps and made me want to sign up. Although I’m not too old to apply, (I have too many attachments at home to do so) if I could do the last ten years over I would apply to their program. I will definitely make sure my students know that the NOAA Corps is an option for them and am hoping to make a trip down to San Diego to take them on one of the boats next year.

I’m particularly grateful to the Chief Scientist Andre DeBose and Watch Leader Taniya Wallace who made sure I knew I was not going to die at sea. As the boat was leaving Galveston I could not stop crying because I was 100% certain I was never coming back ( I may have watched The Perfect Storm too many times). Andre and Taniya were so reassuring and comforting and I can never thank them enough for that.

I’m looking forward to using the knowledge, pictures and data from this trip in my classroom next year. I’m also excited because I heard that I can apply to be a volunteer on a NOAA cruise and am looking forward to this in the future.

July 3, 2014August 21, 2021

Crystal Davis, Female, Male? How do you tell? July 2, 2014

This Common Octopus was found in a 7-Up can.

NOAA Teacher at Sea

Crystal Davis

Aboard NOAA ship Oregon II

June 23-July 7, 2014

Mission: SEAMAP Groundfish Survey

Geographical area of cruise: Gulf of Mexico

Date: Wednesday July 2, 2014

Weather: Clear and sunny with isolated showers and thunderstorms

Winds: 5-10 knots

Waves: 2-3 feet

Science and Technology Log:

Shortly after boarding the Oregon II, the science crew had orientation with the Operations Officer LTJG Thomas reviewing basic procedures for emergencies on board. But what stuck out for me the most, was when Operations Officer LTJG Thomas said we were on a S.A.D. boat. It turns out that S.A.D. means no sex, alcohol or drugs are allowed on the Oregon II. This ensures that the boat is safe and reduces the number of accidents on board. This is the opposite of SAD and makes me feel much safer on board. But luckily for KISS fans, rock and roll is still allowed and is on consistently. Sometimes there’s so much rocking and rolling that I fall on the floor, but that’s happening less frequently as I’ve found my sea legs.

In the Groundfish Survey, after the organisms are separated by species, they are sexed. Overall, this gives the scientists an idea of what future generations will look like. Although all the organisms vary in the way you differentiate their gender, the following are some of the most common organisms found in the groundfish survey.

Sexing Shrimp

Brown Shrimp Female (top) Male (bottom) — Paneaus Aztecas Shrimp Female (top) Male (bottom)

As shown in the pictures on the left, male shrimp have a set of claspers (they look like an extra set of legs) called the petasma that is the equivalent of a penis. Females do not have a petasma.

In young (juvenile) shrimp, it can be difficult to identify the males from females as the petasma is very small and not easily visible. At this age they can easily be confused for females. When this is suspected, they are input into the computer as unknown so as not to generate inaccurate data.

Sexing Crabs

When you pick up a crab you have to be very careful to stay away their claws (cheliped). I have found that they like to grab onto you as soon as you pick them up. My roommate had a large blue crab grab her finger that would not let go and she still has bruises from it.

Mature female crabs are called a “Sook” and have a dome or bell shaped abdomen. This is shown in the top row and looks like the U.S. Capitol Building.

Male crabs are called a “Jimmy” and have a T-shaped abdomen that looks like the shape of the Washington Monument.

To mate, the male crab will carry the female until her shell softens and she is able to mate. During mating, the female stores the males sperm to fertilize her eggs later. Once her shell hardens, the male releases her and she will fertilize her eggs later.

After fertilization, the eggs are stored outside the female’s abdominal area and look like a sponge. They’re very squishy when you touch them. Although this shows orange eggs, they can also be a gray or black color. I have been told that the darker the egg color, the closer to hatching the offspring are. I am not sure that this is scientifically valid and am still trying to verify this.

Sexing Flatfish

Flatfish include fish such as flounder, halibut and turbot. These fish begin their life swimming vertically in the water. However, as they get older they sink to the bottom and their eyes move to one side of their body. They then spend the rest of their life on the bottom of the ocean floor. Luckily their top half matches the ocean floor and they are easily camouflaged from predators. The bottom half of the flounder on the ocean floor is clear or white.

The easiest way to sex a flatfish is to hold them up to a bright light. When doing this you will see that the female has a long curved gonad while the male does not.

A Confused Flounder (right) Normal Flounder (bottom left)

This Flounder is very confused. He should be a clear or light white on the bottom but as you can see his bottom half matches his top half. This could be due to a mutation but no one on the boat is exactly sure why he looks this way. This is one of the most interesting things I have seen so far. In fact, no one on the boat had seen this before.

Sea Jellies

Sea Jellies differ from most of the other marine organisms discussed so far. Sea jellies reproduce both sexually and asexually depending on what stage of life they are in. In an early stage of life sea jellies are called a polyp and they attach to a rock. The polyps reproduce asexually by cloning themselves and breaking off (budding). Imagine 300 people that came from you and look exactly like you. It’s actually pretty creepy. But back to the sea jellies. Eventually the sea jelly will develop into an adult (medusa) that reproduces sexually with sperm and egg.

Personal Log:

I have a three day backpacking trip to Mt. Silliman scheduled almost immediately after my NOAA trip is over. Under normal circumstances I wouldn’t worry, but after spending two weeks not hiking or training, I’m a little concerned. Luckily there are weights and a rowing and elliptical machine on board, so I have been able to do a bit of training. Being on a ship that’s moving has made working out even more intense. I have to stabilize every time the boat moves, so I don’t fall over. But even if I did, or have, how could I complain with this view.

Rowing on the bow

Pelican watching me workout

Frigate

Boat Personnel of the Day

Holland waiting for a trawl to come in — Holland on the stern

Holland McCandless-Lamier

Holland is my roommate on the Oregon II and is a member of the scientific party. She was contracted by Riverside in response to the Deep Water Horizon (BP) blowout in 2010. She attended the University of Mississippi and majored in marine biology. During college, Holland had an internship in Florida where she led students (from 4th grade to college) in marine science activities. This included snorkeling, visiting coral reefs and other hands on activities.

After college, Holland met an individual from the NOAA Corps at a job fair. They put her in touch with NOAA FIsheries MSLabs Groundfish Unit, where she began volunteering as a participant on surveys. This hands on experience led to her current job. Holland currently spends most of her time in the NOAA South East Fishery Science Center (SEFSC) Pascagoula lab where she works with plankton. Her current project is updating decapod (crustacean) taxonomy.

Did You Know?

A female sunfish can lay 300 million eggs each year. Each egg is smaller than the period at the end of this sentence.

June 28, 2014August 21, 2021

Crystal Davis, Bottom Trawl for Shrimp, June 27, 2014

NOAA Teacher at Sea

Crystal Davis

Aboard NOAA Ship Oregon II

June 23 – July 7, 2014

Mission: SEAMAP Groundfish Survey

Geographical area of cruise: Gulf of Mexico

Date: Friday June 27, 2014

Weather: Partly cloudy

Winds: 15-20 knots

Waves: 5-6 feet

Science and Technology Log: Bottom Trawling

The Oregon II is a participant and contributor to SEAMAP (The Southeast Area Monitoring and Assessment Program) which monitors the biodiversity of marine life in the Gulf of Mexico. The primary way the Oregon II assists SEAMAP is by conducting bottom trawls with a 42 foot semi-balloon shrimp trawl net.The net is slowly lowered into the ocean until it reaches the bottom and is then dragged along the ocean floor for thirty minutes. The net has a tickler chain between the doors which scrapes the bottom of the ocean floor and flicks objects into the net. The net is then brought to the surface and all of the organisms inside are put into baskets (see video above). The total weight of the catch is massed on scales on the deck. If the catch is large (over 20 kilos), it is dumped onto a conveyor belt and a random sub-sample (smaller) is kept, along with any unique species while the rest of the catch is dumped overboard.

Once the sample has been selected, the marine organisms are sorted by species and put into baskets. Each species is then massed and counted while the data is recorded into a system called FSCS (Fisheries Scientific Computer System). To obtain a random sampling, every fifth individual of the species (up to twenty) is measured, massed and sexed (more on this later). Once the data has been verified by the watch manager, the marine organisms are put back into the ocean. The following are pictures of a sample on the conveyor belt and the organisms divided into a few species.

Scallops and fish on the conveyor belt waiting to be sorted

The sorting process for shrimp (white, brown and pink) differs slightly from that of the other marine organisms. Every shrimp (up to 200 of each species), is massed, measured and sexed.This data is then used by various government agencies such as the Fish and Wildlife Service, Gulf of Mexico and South Atlantic Fishery Management Councils, etc… to determine the length of the shrimping season and to set quotas on the amount that can be caught by each issued license. States will not open the shrimping season until SEAMAP reports back with their findings from NOAA’s shrimp survey.

The shrimp trawl net used on the Oregon II differs from a shrimp net used on a commercial boat in two main ways. Commercial shrimping boats have BRD’s (Bycatch Reduction Devices) and TED’s (Turtle Excluder Devices). BRD’s and TED’s are federally required in the U.S. to reduce the amount of bycatch (unintentionally caught organisms) and sea turtles. Shrimping boats typically trawl for hours and turtles cannot survive that long without air. TED’s provide turtles and other large marine organisms an escape hatch so that they do not drown (see the video below). Unfortunately, larger turtles such as Loggerheads are too big to fit through the bars in a TED. Additionally, TED’s may become ineffective if they are clogged with sea debris, kelp or are purposefully altered.

Boat Personnel of the Week:

Warren Brown:

Warren is a gear specialist who is working as a member of the scientific party. He is contracted by Riverside for NOAA. While aboard the Oregon II, Warren designs, builds and repairs gear that is needed on the boat. Unfortunately, on this leg of the trip either sharks or dolphins have been chewing holes in the nets to eat the fish inside. This means Warren has spent a large chunk of his time repairing nets.

Warren is not a crew member of the Oregon II and actually works at the Netshed in Pascagoula where he spends his time working with TED’s. He has law enforcement training and will go out with government agencies (such as the Coast Guard or Fish and Wildlife Service) to monitor TED’s on shrimping boats. He also participates in outreach programs educating fishermen in measuring their nets for TED’s, installing them. Warren will bring TED’s and nets to make sure that every everyone at the training has a hands on experience installing them. While he regularly does outreach in Alabama, Mississippi, Florida, Georgia, North Carolina and Texas, his work has also taken him as far as Brazil.

Robin Gropp:

Robin will be a junior at Lewis & Clark College in the Fall. He is currently an intern aboard the Oregon II. Robin received a diversity internship through the Northern Gulf Institute and is one of eight interns for NOAA. For the first two weeks Robin worked at the NOAA lab participating in outreach at elementary school science fairs. He brought sea turtle shells and a shrimp net with a TED installed. The students were very excited to pretend to be sea turtle and run through the TED. They proclaimed, “we love sea turtles.” After leaving the Oregon II, Robin will return to the NOAA lab to study the DNA of sharks.

Personal Log:

Overall I have had a hard time processing and accepting the groundfish survey portion of the trip. I am a vegetarian that does not eat meat, including fish, for ethical and environmental reasons. Yet here I find myself on a boat in the Gulf of Mexico surveying groundfish so that others can eat shrimp. A large part of me feels that I should be protesting the survey rather than assisting. Because of this I spent a lot of time talking to the other scientists on my watch and Chief Scientist Andre Debose. After many discussions (some still ongoing) I do realize how important the groundfish survey is. Without it, there would be no limits placed on the fishing industry and it is likely that many populations of marine organisms would be hunted to extinction more rapidly than they are now. This survey actually gives the shrimp species a chance at survival.

Did You Know?

Countries that do not use TED’s are banned from selling their shrimp to the U.S.

July 16, 2009April 5, 2021

Megan Woodward, July 16, 2009

NOAA Teacher at Sea
Megan Woodward
Onboard NOAA Ship Oscar Dyson
July 1 – 18, 2009

Mission: Bering Sea Acoustic Trawl Survey
Geographical Area: Bering Sea/Dutch Harbor
Date: Tuesday, July 16, 2009

All bony fish have otoliths (ear bones) that can be used for calculating the age of the fish.

Weather and Location
Position: N 58 13.617; W 171 25.832
Air Temp: 7.2 (deg C)
Water Temp: 6.54 (deg C)
Wind Speed: 15 knots
Weather: Overcast

Science and Technology Log

One of the most interesting things I’ve learned while participating in the pollock survey is the importance of otoliths. Otoliths are small bony structures situated in the head of all bony fish, and are often referred to as “ear stones.” For each haul we brought on board, 50 otoliths were taken from large fish (3+ years) and/or 5 from small fish (younger than 3 years old). The otolith holds the key to accurately calculating the age of a fish (scales and vertebrates can also be used, but are not as reliable). The average age of fish from the samples collected in the survey helps scientists estimate the strength of a year-class and size of the stock in the future.

Back in the lab, otolith samples are carefully catalogued.

The first step in taking an otolith is pictured above. An incision is made on the back of the pollock’s head, and an otolith is removed using tweezers. Once the otolith is removed, it is rinsed with water and placed in a glass vial containing a small amount of 50% ethanol solution for preservation purposes.

The otoliths are taken back to NOAA’s aging lab where ages are determined by reading rings similar to those on a tree trunk. A crosscut is made through each otolith revealing a pattern of rings. Scientists then count the rings to determine the age of the fish. Lightly burning or staining the otoliths makes the rings more visible.

New material is deposited on the surface of the otolith creating the rings as the fish grows. The translucent/light zones indicate the main growth that takes place in the summer months. The opaque/darker rings appear during the winter months when growth is slower. Because of the slower growth rate, new material is deposited on top of the old layers resulting in the dark ring. Each pair of light and dark zones marks one year. In fish younger than one year of age, rings can be identified for each day of life!

Personal Log

I was surprised to discover otoliths have been used for aging fish since the early 1900’s. While working in the fish lab I observed the scientist removing otoliths, however I did not remove any myself. The cracking sound heard when cutting the head open was like fingernails on a chalkboard to me. I spent most of my time in sorting and measuring fish, as well as assisting with the stomach collection project.

For the next two days we will be heading back to Dutch Harbor, and the likelihood of trawling for more fish is minimal. Our remaining work assignment is to give the fish lab a thorough cleaning. Everything in the lab is waterproof, so we’ll put on our Grunden’s (orange rubber coveralls) and boots and spray down the entire space. Working and living at sea for nearly 3 weeks has been an eye opening experience. My time aboard the Oscar Dyson has flown by. I have learned so much about fisheries research and life at sea. Dry land, however, will be warmly welcomed when we get back to Dutch Harbor. Would I do it again? Absolutely.

Animal Sightings

The whales have an incredible way of showing up when I don’t have my camera. Yesterday I spotted two orcas, but did not get a photograph. The seabirds continue to circle. I like the murres most. They look like small, flying penguins.

New Vocabulary

Otoliths- Small bony structures situated in the head of all bony fish. Often referred to as “ear stones.”

Stock- Refers to the number of fish available, supply.

*** Much of the information used for this log entry was found on the Centre for Environment, Fisheries & Aquaculture Science (Cefas) web site.

July 12, 2009April 5, 2021

Megan Woodward, July 12, 2009

NOAA Teacher at Sea
Megan Woodward
Onboard NOAA Ship Oscar Dyson
July 1 – 18, 2009

Mission: Bering Sea Acoustic Trawl Survey
Geographical Area: Bering Sea/Dutch Harbor
Date: Tuesday, July 12, 2009

Any bycatch in a haul has to be measured and weighed if there are more than 25 of the same species caught. — Any bycatch in a haul has to be measured and weighed if there are more than 25 of the same species.

Weather/Location
Position: N 60.35.172; W 174.08.187
Air Temp: 6.1 (deg C)
Water Temp: 5.24 (deg C)
Wind Speed: 25 knots
Weather: Overcast, rain

Science and Technology Log

How is all the data collected from a trawl and acoustic lab used? By collecting data about weight and length from a sample, scientists are able to connect the size of fish caught to the amount of return seen in the acoustic lab. The return is assigned a name (PK1, PK2, etc.) and all schools showing a similar acoustic pattern are given the same name. In the end, scientists can estimate the number of fish and their size for a given area based on the acoustic and fish lab data collected. This is repeated throughout the survey resulting in an estimate for the total number of fish in the survey area.

Both during and after the survey estimates of abundance in the same location over the past several years are compared. Scientists evaluate the data and determine if the pollock population in the survey area is increasing, declining or stable. Their conclusions are used to make a recommendation about pollock fishing limits for the upcoming year. In the past few years the pollock population has been lower than in previous years. Due to the decline, the fishing quota has been reduced. However, the 2006 year-class is proving to be strong. At 4 years of age pollock are considered mature and fishable. Therefore, the fishing quota is predicted to rise in the next year or two.

Personal Log

While discussing the acoustic survey project with the scientists on board, I was quite surprised to hear the pollock survey had been going since 1979. Acoustic technology has changed and improved, but in essence the project has remained the same. Modern computer technology has allowed collection and analysis of enormous data sets and greatly reduced the amount of paper work needed for the project’s success.

The concept of strong vs. weak year-class is also quite interesting. There doesn’t seem to be a direct connection between a year-class’ success and environmental factors. Environmental factors that are potentially influential are water temperature, available zooplankton, ice cover, storms and predators. The fish currently being caught by commercial fisherman are 5-7 years old. Can you figure out which year classes those fish are from?

We continue to spot plenty of seabirds and a few more minke whale pods. I was able to watch a group of Dall’s porpoises play in the wake of the bow for half an hour yesterday. There haven’t been any new animal sightings during the past few days.

Although we are out here working in the best interest of pollock, I have found it difficult to watch thousands of pollock come through the fish lab. I have to remind myself that sampling the fish is truly for the good of the order. In addition, after being measured the fish are sent back into the ocean where they become food for other organisms such as crab or birds. One of their natural predators is having a good meal, something that was likely to happen anyway.

Animal Sightings

Seabirds
Dall’s porpoises

New Vocabulary

Bycatch – Anytime something is caught during a trawl other than pollock it is labeled bycatch. Jellyfish has been the most common form of bycatch.

Year-class – All the fish born in a given year are members of that year-class. We have caught a lot fish from the 2008 year-class (1 year old fish).