dissection – NOAA Teacher at Sea Blog

July 13, 2023July 19, 2023

Lisa Carlson: Where Did You Come From, Where Did You Go? July 13, 2023

NOAA Teacher at Sea

Lisa Carlson

NOAA Ship Bell M. Shimada

July 5, 2023 – July 19, 2023

Mission: Fisheries: Pacific Hake Survey (More info here)

Geographic Region: Pacific Ocean, off the coast of California

Date: July 13, 2023

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Weather Data from the bridge:

July 11 (1200 PT, 1500 EST)
Location: 37° 46.7’ N, 123° 26.6’ W
43nm (50mi) West of San Francisco, CA

Visibility: 2 nautical miles
Sky condition: Overcast, fog
Wind: 20 knots from N 250°
Barometer: 1015.2 mbar
Sea wave height: 2-3 feet
Swell: 6-7 ft from NW 320°
Sea temperature: 12.2°C (57.2°F)
Air temperature: 12.7°C (57.9°F)
Course Over Ground: (COG): 270°
Speed Over Ground (SOG): 10 knots

July 12 (1200 PT, 1500 EST)
Location: 38° 06.8’ N, 123° 01.6’ W
7nm (8mi) North of Point Reyes Lighthouse, Inverness, CA

Visibility: 2 nautical miles
Sky condition: Overcast, fog
Wind: 12 knots from N 350°
Barometer: 1016.0 mbar
Sea wave height: 1-2 feet
Swell: 3-4 ft from W 280°
Sea temperature: 11.0°C (57.2°F)
Air temperature: 11.5°C (57.9°F)
Course Over Ground: (COG): 270°
Speed Over Ground (SOG): 10 knots

July 13 (1200 PT, 1500 EST)
Location: 38° 17.3’ N, 123° 06.1’ W
2.5nm (4mi) Southwest of Bodega Bay, CA

Visibility: 3 nautical miles
Sky condition: Few clouds, fog
Wind: 13 knots from NW 300°
Barometer: 1015.9 mbar
Sea wave height: 1-2 feet 1-2
Swell: 3-4 ft from NW 300°
Sea temperature: 10.7°C (51.3°F)
Air temperature: 13.7°C (56.6°F)
Course Over Ground: (COG): 340°
Speed Over Ground (SOG): 10 knots

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In my July 6 post, I explained how NOAA Ship Bell M. Shimada is equipped to collect acoustic data in the form of echo grams and therefore find fish to trawl for. In my July 10 post, I explained how we get the fish onboard, and what we do with the sample once it is collected from the net. These entries described what work is done in the Acoustics Lab and the Wet Lab, but there is one more Lab onboard to explore and explain: the Chemistry Lab.

view down the starboard side of NOAA Ship Bell M Shimada shows a wooden nameplate (reading Bell M Shimada) on a railing, the fast rescue boat mounted aftward, and the Golden Gate Bridge in the background. — NOAA Ship *Bell M. Shimada* leaving Pier 30/32 in San Francisco, CA on July 5, 2023. (Just a nice photo taken by me that I wanted to include)

Science and Technology Log

Each morning after breakfast, we usually gather in the Acoustics Lab, determine what transect we are on, if we are inshore or offshore, and in some ways: hurry up and wait. Once certain patterns and blips show up on the echo grams, the Acoustics team talks with the bridge and may request to turn around and attempt a trawl. After all marine mammal observations are completed, the net is retrieved, and the samples are brought to the Wet Lab, we sort and collect data on the samples. These operations usually take place between 0800 and 2000. (8am to 8pm)

So what happens at night? In the Chemistry Lab, scientists work with the Deck and Surveys Departments to deploy a collection of electronic instruments and 12 Niskin bottles (open bottles used to collect and hold water samples, about one meter long) secured to a cylindrical frame called a rosette. It is deployed from the side sampling station instead of the stern. Scientists onboard NOAA Ship Bell M. Shimada use the instruments and collection of water samples in two ways: measuring Conductivity, Temperature, and Depth (CTD) within a water column to study oceanography, and collecting environmental DNA (eDNA).

photo of a large piece of sampling equipment on deck. a large white metal cylindrical frame houses a ring of perhaps ten tall gray canisters - the Niskin bottles. The bottles circle the conductivity, temperature, and depth probe, which is barely visible. Behind the frame, past the ship's rail, we see vivid blue water with a few white caps and a coastal mountain range beyond.

CTD Niskin bottles arranged on a circular rosette frame.

“Nighttime operations primarily consists of deploying the Conductivity-Temperature [-Depth] (CTD) rosette which gathers oceanographic data such as conductivity, temperature, dissolved oxygen, and chlorophyll fluorescence. The CTD can also be triggered to collect water at specific depths.”
NOAA Fisheries: “eDNA Part 2: There’s a Lot of Water in the Sea – and the Chemistry Lab”

NOAA Ocean Exploration: “What does “CTD” stand for?”

Conductivity, Temperature and Depth: CTD

CTD stands for conductivity (ability to pass an electrical current), temperature, and depth. Scientists use the rosette frame, which is attached to the ship by cables, and has the CTD and 12 Niskin bottles attached, to collect electronic data and multiple water samples.

“A CTD device’s primary function is to detect how the conductivity and temperature of the water column changes relative to depth. Conductivity is a measure of how well a solution conducts electricity and it is directly related to salinity. By measuring the conductivity of seawater, the salinity can be derived from the temperature and pressure of the same water. The depth is then derived from the pressure measurement by calculating the density of water from the temperature and the salinity.”
NOAA Ocean Exploration: “What does “CTD” stand for?”

Senior Survey Technician Elysha Agne gives commands to the Deck Department running the winch and cable to the rosette, and ensures quality data is being collected at each sampling depth.

“For more detailed analyses back in the lab, each of the large gray bottles captures a water sample at a different depth. The data provide scientists important information about the local aquatic environment.”
NOAA: “Photo story: Virtually cruise aboard a NOAA ship for a fish trawl survey”

Depending on the depth at which the vessel is currently operating, the rosette will descend to one to five predetermined depths (50m-500m) for sampling. For example, if the vessel depth reads 400m, water samples will occur at 50m, 150m, 200m, and 300m (more information in Table 1 below). A water sample is also taken just below the ocean surface using a through hull fitting, which allows seawater to be collected via a hole in the hull that feeds directly to the Chem Lab.

Table 1. Sample depths for eDNA. Two independent samples should be taken at each depth. The total ocean depth of location for the CTD cast determines the depths at which water samples will be collected. The rows of the table are labeled Sampling Depth (m) and the columns are labeled Topography depth of CTD cast. — Table 1 in Protocol manual, written by Chem Lab member and eDNA scientist Abi Wells.

While the rosette descends, data is recorded from multiple sensors and are later used by scientists to compare with Acoustic and Wet Lab data and compile and categorize new information from the survey. Pressure, depth, temperature, conductivity, salinity, oxygen, fluorescence, and turbidity were all being recorded during this leg of the survey mission.

photo of a computer screen displaying data. two graphs depict depth (m) on the y-axis and salinity or dissolved oxygen on the x-axes. — Program displaying data collected from the CTD rosette in real time.

Environmental DNA: eDNA

During the day, Hake stay in deeper waters, averaging around 200-350m, but at night the nocturnal feeders start their daily migration through the water column to shallower depths. They feed primarily on zooplankton, shrimp, myctophids (Lanternfish), and even young Hake at this depth. As Hake move throughout the water column, they leave behind DNA in the water that can be collected later as sort of a signature of their presence in that location. The collection, filtering, and preservation of sampled water in the ocean environment is categorized as collecting eDNA. This environmental DNA can be in the form of gametes (reproductive cells), fish scales, feces, etc.

Collecting water samples at different depths in the same vertical column can show what marine life was present at that location, and what depth they were at. I relate it to reviewing school security cameras or talking to other teachers at the end of the school day, to determine where a student was at a certain time and why.

Chem Lab member and eDNA scientist Abi Wells collecting a 2.5L water sample from a Niskin bottle after a successful CTD deployment.

When the rosette is back on deck, scientists use gloves and new collection bags called Whirlpacks, to collect approximately 2.5L of water from each 10L Niskin bottle. This process is conducted with a great emphasis on sterility, including wiping the bottle spigot with DNAway to remove any contaminants, using new materials, and not allowing fingers or the spigot to touch the collection bag.

White paper is taped down on a bench in the wet lab. On it are rows of water filtering cups mounted on gray horizontal pipes attached to hoses. Each filter cup is topped with a paper filter. Two have funnels attached; additional funnels, still sealed, line the workbench behind the filtering cups. — Sterile work site being set up to pour water samples into the cups and strain through the filters.

blue gloved hands pour water from a clear plastic bag into a funnel attached to a filtering cup. nearby, three plastic pitchers serve as holders for open plastic bags will filled with water. — Sterile work site being set up to pour water samples into the cups and strain through the filters.

Once the collection bags are filled and brought to the Chem Lab, filtration occurs using 1.0 micron filters. Although this size of filter, compared to smaller filters, allows some cells to pass through and not be collected, it is faster and results in less breakage of cells and loss of DNA. After 2.5L of the water sample is poured through individual filters for each depth sample, they are placed in pre-labeled (location and depth information) tubes with 2mL of preservative buffer. The tubes are stored at room temperature and away from UV light until NOAA Ship Bell M. Shimada is back in port and the samples can be further researched in on-land laboratories. Results from additional studies help to compile lists of marine life that was present in the water column and can be compared to acoustic data and species caught and logged in the Wet Lab.

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Personal Log

So, there you have it. Three Labs onboard that conduct very different research, but fit together in the puzzle of Hake development, migration, diet, niches, ecosystem, biomass, and supporting sustainable commercial fisheries. Each additional piece of data; whether it be echo sounds, physical samples, eDNA, or CTD information, strengthens the others and helps to create a cohesive summary of the data.

This was a lot to learn in the first few days, but as I’ve said before, all of the crew has been welcoming, supportive, and educational. Having a strong team that works together is priceless, and thoroughly noticed and appreciated.

A few days into the mission my Mom asked me what the best part of my day was. I had three answers and haven’t had a day yet with only one answer. I replied that it was the great salmon dinner, clean clothes, and seeing Risso’s Dolphins for the first time.

Video taken by me of Risso’s Dolphins surfacing for air. (Plays on loop)

We are now a little more than halfway through the mission and it has truly flown by. We’ve shared riddles and daily Final Jeopardy questions. We’ve laughed over daily experiences and the faces Hake fish make. We’ve played music and watched baseball during dinner. We enjoy watching marine life and breathe in the salt air while strengthening our sea legs. Sometimes we just drink coffee and snack and enjoy this opportunity with each other, and that makes every part of the day the best part.

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Did You Know?

Although Hake are occasionally cannibalistic, they are not at the top of their food chain. Humboldt Squid (Remember those 15 foot long tentacles in my Wet Lab post?), Dogfish Sharks, and marine mammals are all predators, as well as commercial fishing.
Today well over 100 Spiny Dogfish Sharks were inadvertently caught in the trawl, in the same location as the baskets of Hake we sampled from.
Maybe there were baby Hake fish in the sharks’ stomachs… we didn’t attempt to find out.

a white plastic basket of small sharks — Basket one of Spiny Dogfish

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New Terms/Phrases

Although I had learned the terms a few days earlier, I got to help Wet Lab Lead Ethan Beyer collect otolith and stomach samples for the first time from a sub-sample of Hake the other day.

I watched and learned, then helped scan barcodes of otolith sample bottles, add 95% ethanol that is diluted 50/50 with water, and delicately pick up the ear bones with tweezers and place them in the bottle.

Additionally, each Hake in the sub-sample has its weight recorded, along with length, sex, and developmental stage. From that sub-sample, five stomachs are removed for later analysis, and five have their stomachs opened and their diet is recorded. We often find Lanternfish (Myctophids), Krill (Euphausiidae) and small Hake.

Two orange-gloved hands reach toward a fish on an electronic scale. the ichthystick measuring board is on the table in front of the scale. To the right, in the foreground of the photo, is a large styrofoam case holding small glass sample bottles upright. about two thirds are capped with black caps, while the rest are empty. — Wet Lab Lead Ethan Beyer weighing a Hake sample. Otolith tubes are in the foreground, those with lids have samples inside them and have been scanned into the database with other measurements and samples data from the Hake.

July 10, 2023July 19, 2023

Lisa Carlson: One Fish, Two Fish, Rockfish, Hake fish! July 10, 2023

NOAA Teacher at Sea

Lisa Carlson

NOAA Ship Bell M. Shimada

July 5, 2023 – July 19, 2023

Mission: Fisheries: Pacific Hake Survey (More info here)

Geographic Region: Pacific Ocean, off the coast of California

Date: July 10, 2023

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Weather Data from the bridge:

July 7 (1200 PT, 1500 EST)
Location: 36° 00.4’ N, 122° 05.9’ W
16nm (21mi) West of Big Sur, CA

Visibility: 10 nautical miles
Sky condition: Overcast
Wind: 20 knots from NW 330°
Barometer: 1013.1 mbar
Sea wave height: 3-4 feet
Swell: 6-7 ft from NW 320°
Sea temperature: 14.0°C (57.2°F)
Air temperature: 14.4°C (57.9°F)
Course Over Ground: (COG): 323°
Speed Over Ground (SOG): 10 knots

July 8 (1200 PT, 1500 EST)
Location: 36° 34.5’ N, 122° 05.3’ W
17nm (20mi) Southwest of Monterey, CA

Visibility: 10 nautical miles
Sky condition: Few clouds
Wind: 19 knots from NW 330°
Barometer: 1013.8 mbar
Sea wave height: 5-6 feet
Swell: 6-7 ft from NW 330°
Sea temperature: 14.0°C (57.2°F) 13.7
Air temperature: 14.4°C (57.9°F) 14.3
Course Over Ground: (COG): 089°
Speed Over Ground (SOG): 10 knots

July 9 (1200 PT, 1500 EST)
Location: 37° 06.8’ N, 123° 00.5’ W
30nm (35mi) West of Pigeon Point Light Station, Pescadero, CA

Visibility: 10 nautical miles
Sky condition: Overcast
Wind: 13 knots from NW 332°
Barometer: 1016.0 mbar
Sea wave height: 2-3 feet
Swell: 4-5 ft from NW 310° 4-5
Sea temperature: 14.3°C (57.7°F)
Air temperature: 15.2°C (59.4°F)
Course Over Ground: (COG): 093°
Speed Over Ground (SOG): 10 knots

July 10 (1200 PT, 1500 EST)
Location: 37° 26.7’ N, 123° 06.4’ W
32nm (37mi) West of Pescadero, CA

Visibility: 8 nautical miles
Sky condition: Overcast, fog in vicinity
Wind: 20 knots from NW 330°
Barometer: 1015.9 mbar
Sea wave height: 2-3 feet
Swell: 3-4 ft from NW 320°
Sea temperature: 14.5°C (58.1°F)
Air temperature: 13.6°C (56.5°F)
Course Over Ground: (COG): 314°
Speed Over Ground (SOG): 3 knots

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Science and Technology Log

Lisa poses for a photo in the wet lab with a hake fish. She's wearing heavy-duty orange overalls and large orange gloves. With her right hand, she grasps the fish by its open mouth, and her left hand holds on to the tail. We can see metal tables and equipment in the background. — Me holding a Hake before sorting. After observation, we determined this was a developmentally mature female, measuring 50cm (20in) long!

In my July 6 blog post, I explained how NOAA Ship Bell M. Shimada is equipped to collect acoustic data in the form of echo grams. The acoustics team uses the data to determine if there are enough return signals to suggest fish are present and attempt a trawl. In this blog post, I will explain how we get the fish onboard, and what we do with the sample of marine life once it is collected from the net.

One question I had after learning about the acoustics and environmental DNA (eDNA) pieces of the survey mission was, “How does physically collecting and researching Hake samples fit into the puzzle of understanding their ecosystem and supporting sustainable fisheries?” (NOAA Fisheries quick facts and video here)

“While echosounders are useful, they do not provide certain quantitative data that researchers need to understand the ecology of these organisms and the midwater zone. To collect quantitative data, such as biomass, length and weight, and age class distributions, researchers must gather representational samples and take direct measurements of them. The best way to do this is by employing trawls.”
NOAA Ocean Exploration: “Trawls”

So, although acoustics and eDNA research is important to the overall survey, they are only pieces of the puzzle, and the puzzle is not complete without conducting trawls and physically researching samples. NOAA Ship Bell M. Shimada uses a midwater trawl net that is deployed from the stern over the transom, and towed behind the vessel. As the name suggests, midwater trawls occur in the middle section of the water column, versus surface and bottom trawls. The net is conical in shape and uses two metal Fishbuster Trawl Doors, and two sets of heavy chain links called Tom weights, in order to keep the trawl in the middle of the water column.

a simple and stylized monochrome illustration of a fishing vessel towing a midwater trawl behind it. The net in tow is conical, attached at four points to two bars that hold the opening apart, and these bars are attached to lines (ropes) extending back from the vessel. This net is capturing two fish and missing a third. — NOAA Fisheries: “Fishing Gear: Midwater Trawls”

“The midwater region is especially important because the creatures that inhabit it constitute the majority of the world’s seafood. Understanding the ecology of midwater organisms and their vast environment can provide us with better information to manage these important natural resources and prevent their overexploitation.”
NOAA Ocean Exploration: “Trawls”

Deck department assisting in recovering the trawl net after a successful deployment.

Two deck crewmembers work with an orange and white fishing net on the aft deck of NOAA Ship Bell M. Shimada. They are wearing foul weather gear, life vests, and hard hats. At right, one leans over the net, searching for remaining captured fish. The other approaches from the left, looking down at the net, to assist. We can see a cloud-capped mountain range in the distance beyond the water.

Once the net is onboard, the net is emptied one of two ways depending on the size of the sample. For large samples, marine life is deposited into a hopper and subsequent conveyor belt. For smaller samples, the Hake will be put into a large basket then divided into smaller baskets of approximately 100 Hake each. Any other marine life like Salps, Myctophids, Pyrosomes, Rockfish, King of the Salmon, and small bony fish, etc. are recorded in the database and returned to the ocean.

“The ship’s wet lab allows scientists to sort, weigh, measure and examine fish. The data is entered directly into the ship’s scientific computer network.”
NOAA Office of Marine and Aviation Operations (OMAO): “Bell M. Shimada”

a large black plastic bin filled with fish - mostly hake, but a few splitnose rockfish (eyes bulging from the pressure change) stand out for their red color. An orange-gloved hand reaches toward the basket from the upper left corner of the image.

Large basket containing a sample of Hake with a few (red) Splitnose Rockfish.

a view of NOAA Ship Bell M. Shimada’s Wet Lab with multiple scales, Ichtystick electronic measuring boards, trawl camera, vials for otolith (ear) bones, disposal chute, and tools including scalpels, tweezers, and knives. — NOAA Ship *Bell M. Shimada’s* Wet Lab with multiple scales, Ichtystick electronic measuring boards, trawl camera, vials for otolith (ear) bones, disposal chute, and tools including scalpels, tweezers, and knives.

Maddie, in the wet lab, holds a hake fish up for a photo. She is wearing a long-sleeved shirt, heavy-duty orange overalls, and large orange gloves. Her hair is tied back in two double braids and topped with a teal bandana. She holds the fish in two hands, horizontally, at the level of her mouth, so we cannot see her smiling, but we can see her arch her eyebrows as she looks straight at the camera. — NOAA Ship *Bell M. Shimada’s* Wet Lab with multiple scales, Ichtystick electronic measuring boards, trawl camera, vials for otolith (ear) bones, disposal chute, and tools including scalpels, tweezers, and knives.

With our boots and bright orange rubber pants and gloves on, our first task is to distribute the sample of Hake into baskets of about 100 each. Based on how many baskets we fill, a random selection of baskets will be kept, and the others will be returned to the ocean. With the remaining groups of Hake, we determine their sex and length.

In order to do this, we use a scalpel to make an incision on the underside/belly of the Hake. Once open, we are able to examine their organs, including the gonads to determine if the fish is male or female, and if they are developmentally immature or mature. Young Hake are difficult to sex, and it takes practice to get over any initial fears of cutting into an animal; let alone being able to locate and identify the gonads. Hake usually spawn in early winter, so many of the smaller Hake we sample from during the summer are age one or younger.

Our largest Hake thus far was a developmentally mature female, measuring 50cm (20in). In order to accurately and consistently measure the length of the sample, we use a waterproof, magnetic plastic board with metric (centimeter and millimeter) markings called an Ichthystick (think: high-tech meter stick). The fish is placed on the board with its mouth touching the black board at 0cm, then a magnetic stylus is placed at the fork of the fish’s tail. Once the magnetic stylus is placed on the board, the length to the nearest millimeter is displayed on the LCD screen and automatically entered into the database program. The length data is grouped with the date, time, and identified sex for later observation and comparison.

Additional information, abstracts and outline about Ichthystick here

Ichthystick’s LCD display, motherboard, magnetic board, and magnetic stylus. Digital scale in background.

An even smaller subgroup is then selected and examined to record weights of individual Hake, collect ear bones called Otoliths for aging, stomach samples for diet, liver for RNA, and ovaries for maturity development. Otolith bones help determine the age of the Hake because they grow a new “layer” of bone each year, similar to coral structures and annual tree rings. Organs and bones removed from the Hake are sent to NOAA Fisheries centers for analysis and included in databases with the date, identified sex, length, weight, and location in which they were collected.

This data is used to build more of the puzzle, along with acoustical information, water samples, and eDNA data in order to further understand the ecosystem, biomass, diet, and

“support sustainable populations of Pacific hake on the West Coast.” (…)
“It provides vital data to help manage the migratory coastal stock of Pacific hake. The hake survey, officially called the Joint U.S.-Canada Integrated Ecosystem and Pacific Hake Acoustic Trawl Survey, occurs every odd-numbered year.”
NOAA Fisheries: “Joint U.S.-Canada Integrated Ecosystem and Pacific Hake Acoustic Trawl Survey”

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Personal Log

Although this subtopic of explaining the Integrated Ecosystem and Pacific Hake Acoustic Trawl Survey is a bit easier to understand than my July 6 Acoustics Lab post, it certainly does not mean it’s an easy task!

When I had a tour on July 4, I remarked how clean and organized the Wet Lab is. I hadn’t see it in action yet, but noticed how everything had its place and use. On July 6 we conducted our first trawl and collected a sample of 11 baskets of Hake (approximately 1,100 Hake since we group about 100 Hake together in each basket.) From that sample, we kept four baskets and counted, sexed, and measured 541 Hake.

Five of us were working together in the Wet Lab for that haul. I’ll admit I probably didn’t sex 100+ Hake. It took a few minutes of watching the others carefully and swiftly cut into the underside of a fish, open the two sides, and know what to look for to determine the sex of very young Hake. Eventually I found the courage to slice in and take a look. By the fourth or fifth Hake, the uneasiness had subsided and I found the process very interesting and educational. Although young samples are hard to sex as they are often undeveloped, the others encouraged me and answered my questions and guesses with enthusiasm and support.

While working on measuring the lengths of our samples, one Science Team member paused and remarked how beautiful he found the fish. Although they do not have vibrant, bold colors, shimmering scales, or anything else particularly remarkable, he found the beauty in them. He digressed into a conversation of their role in the ecosystem, how they are living and breathing creatures, and how they probably all have their own personalities and slight physical differences. I noticed some of their eyes were shiny and sparkling, and how their faces and expressions were noticeably unique the more you looked. That “down to earth”, heartfelt discussion was very special and demonstrated how the crew respects the process of catching and sampling Hake, while keeping each other and marine mammals safe.

From the NOAA Corps Officers, to the deck department, to the engineers, electronics, science team, survey team, galley crew, volunteers, and everyone in between; the crew on NOAA Ship Bell M. Shimada is special. They take pride in their vessel and job, and always seem to have a smile and kind greeting. Being away from land and loved ones for weeks and months at a time will certainly take a toll on the body and mind, but this team is there for each other. To all of the crew, thank you for making me feel so welcomed and appreciated. We’re almost halfway through the mission, and as tired as I may get after (sometimes) 12+ hour days, I sleep well knowing the crew trusts their vessel and each other; and look forward to learning and becoming more and more acquainted each day with the people that make this mission possible. Thank you!

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Did You Know? (FAQs)

1. Are you finding schools of them?

We’ve had seven successful trawls out of nine attempts for Pacific Hake fish. They often come with pyrosomes (Sea Pickle) myctophids (Lanternfish), and salps in the net too. Some trawl attempts are successful without a hitch, but more often than not we have to restart our Marine Mammal watches a few times before deploying in order to keep our ocean life safe and not get tangled in the net. Two trawl attempts have been abandoned because of the amount of persistent marine mammal life and playfulness near the ship. (I think they know we’re watching and show off for our cameras.)

2. What’s your average depth?

The transects (Set and numbered longitudinal east-west lines NOAA Ship Bell M. Shimada navigates on while collecting acoustic data) usually range from 50m – 1,500m (164ft – 4,921ft) in depth.

However, right now one of the displays in the Acoustics Lab, the depth reading is 3,240m which is about 10,630ft or just over two miles deep!
This depth is only 1,870ft shallower than the wreck of the RMS Titanic!
(We were on a long transect, we do not often see depths this great.)

3. Have you gotten seasick? Seasickness should subside after about 3 days.

I’ve never gotten seasick thankfully! Knock on wood and all the other premonitions, please.

4. What is the Hake role in the ecosystem?

More info on this coming in later posts after explaining our Chemistry lab and technology aboard!

However, as predators, they can be cannibalistic towards their own kind.
As far as their role in human consumption: They are often used as a substitute for Cod and Haddock, and in fish sticks and imitation crab meat.

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Animals seen July 5-July 10:

Mammals: Sea Lions, Harbor Seals, Dall’s Porpoise, Risso’s Dolphins, Pacific White-Sided Dolphins, Northern Right Whale Dolphins, Humpback Whales

Birds: Gulls, Black-Footed Albatross

Bony Fish: Hake, Lanternfish (Myctophid), Flatfish, King of the Salmon, Split Nose Rockfish, Chili Pepper Rockfish

Other Marine Life: Giant or Humboldt Squid (15 foot tentacles in trawl), Spiny Dogfish Shark, Shrimp, Plankton, Krill, Sea Pickle (Pyrosome), Salp, Eel Larva

a spiny dogfish - a species of small shark - placed on a fish measuring board atop a metal table. a scale is visible next to the measuring board. — Spiny Dogfish Shark (42cm or 16in long)

three impressively long squid tentacles laid out on the floor; they are orange-brown on top, and white underneath, lined with white suckers (suction cups) of varying size — Spiny Dogfish Shark (42cm or 16in long)

June 21, 2022June 21, 2022

Jordan Findley: Fishing, June 20, 2022

NOAA Teacher at Sea
Jordan Findley
Aboard NOAA Ship Pisces
June 9-22, 2022

Mission: SEAMAP Reef Fish
Geographic Area of Cruise: Gulf of Mexico
Date: June 20, 2022

Science and Technology Log

Fishing Operations

Alright, it’s time for the good stuff, the moment you’ve been waiting for (whether you knew it or not). It’s fishing time. FPC Paul Felts monitors depth and habitat to determine suitable fishing sites. When the crew hears “I’d like to set up for bandit reels” over the radio, they come running. I mean they come out of the woodworks like the Brady Bunch on Christmas morn. Let me remind you, the days can be real slow out here. Lots of transiting and waiting. Fishing offers just enough excitement to keep us going.

Three bandit reels are deployed once or twice per day. I promptly insert myself into the fishing operation on day one. Thank you, Rafael and Junior. The reels are motorized and mounted to the side of the ship. The line starts with a weight and then ten baited hooks are clipped on. When deployed, it sinks to the bottom. We get five minutes. Five short minutes for the fish to bite. Boy does anticipation build in that five minutes. If you have a good one, you can feel it on the line. “One minute to haul back.” By this time, everyone is leaning over the side (the gunwale if you want to be fancy) staring at the water. “Reels two and three you can haul back.” “Reel one you can haul back.” We start reeling back in, from somewhere between 85-100 meters deep. Click, click, click on the reel as we impatiently wait.

a crewmember wearing a life vest reaches over the railing on the side of the ship to bait the line on the bandit reel — Baiting the line

several crewmembers look over the side railing, waiting — Baiting the line

We start to see a glimpse of the bait coming up around 40-60 meters and try to make out what we’ve hooked. RED SNAPPER! 11 red snapper caught between the three reels on the first fish. This is what I’m talkin’ about. I can handle two weeks of this. Everyone rotates between stations to see what we caught and we all celebrate like we just won some sort of tournament. Let’s remember folks, we are doing this for science. All fish captured on the bandit reels are identified, measured, weighed, and have the sex and maturity determined. Select species have otoliths and gonads collected for age and reproductive research. I excitedly follow the science crew into the lab to get the run down.

in the wet lab, Caroline, wearing a life vest, holds up two red snapper (one larger than the other) by the lines of the hooks in their mouths — Caroline with red snapper

on deck, Amanda holds a marbled grouper by its gill rakers — Caroline with red snapper

on deck, Jordan holds up a hooked marbled grouper by the line — Marbled grouper

Jordan holds a queen snapper up for a photo; she is holding it toward the camera to create a forced perspective — Marbled grouper

*Read no further if you are squeamish.*

The work up of the fish start with some measurements and weights. Of course it immediately became a competition. Game on. Now these fish aren’t your regular ol’ fish. These suckers are huge. Next we dissect the fish to extract and weigh the gonads. That’s right, I said gonads. You can learn the age and maturity of a fish by examining a sample of the gonads under a microscope. From that, you can estimate lifespan, spawning patterns, growth rate, and possibly even migration patterns. Knowing the age distribution of a fish population helps to better monitor, assess, and manage stocks for long-term benefits. Fish gonads, that’s a first for me.

Next step is the fun part, extracting the otolith. Otoliths (ear bones) are calcium carbonate structures found enclosed inside the heads of bony fish. This bone tells us how old the fish is. Otoliths are removed from the fish’s head either by entering through the top of the head or by pulling back the gills. At first, I observe. They really get in there. By the third or so time, I am ready to get my hands dirty. Remove the gills and start digging. Once you find the inner ear, you crack it open and inside is the otolith. Some species are much easier than others. It’s no walk in the park folks. One grouper took us two hours. It’s like a real life game of operation. Though intense, it’s a fun challenge.

Jordan measures a snapper on a fish measuring board in the wet lab — Measurements

Caroline, wearing a life vest and gloves, records data on a data sheet in the wet lab — Measurements

a data sheet for recording lengths — Data sheet

On this leg of the survey we caught 20 red snappers, 2 silky snappers, 1 queen snapper, 2 scamp, 1 marbled grouper, 1 yellow edge grouper, and 1 red porgy. Sampling these organisms strengthens the data. Employing multiple research methods produces a comprehensive description and interpretation of the data. The workup of the fish was one of my favorite parts of this experience. Not only did I actually get to participate in the research, I learned valuable new skills, most of which I teach about, but have never had the chance to do it. This is the exact reason I applied for the Teacher at Sea Program.

Have I convinced you that science is cool yet?

Meet the Deck Crew

I’d like to give a shout out to my friends on the deck. NOAA Ship Pisces couldn’t do the research they do without the Deck Department – Chief Boatswain James, Lead Fisherman Junior, and ABs Dee and JB. The Deck keep up general maintenance of the boat and on deck, operate equipment and machinery, support scientific operations, and stand watch. These guys might be salty, but they have good spirits and make me smile. I have enjoyed every minute working with them.

JB on deck, wearing a life vest, holds up a hooked fish — AB Jamison “JB” Boyd

Junior, wearing a life vest, hard hat, and sunglasses, carries a bucket or trash can somewhere — AB Jamison “JB” Boyd

Personal Log

Yesterday, we did another fire drill. This time, with the help of firefighter Jordan Findley. LT Duffy set me up to participate in the drill. He shows me the gear and how it works. It’s hot up in there. Two days later when the alarm sounds, I jump to attention. Not really. It took me a minute to remember I was involved. I pop up out of my usual lounging in the lab and swiftly head out to the deck. 0% do I remember where I am supposed to go. Thank god I pass JO ENS Gaughan. She points me in the right direction. By the time I make it to the locker, they’re all dressed out and on their way to “fight the fire.” They’re impressive.

Though late to the game, JB helps me get suited up and I head down to the scene. As you might expect, the “fire” is out by the time I arrive. I provided moral support. Following the drill, we (I trail behind and try not to trip) walk the hose outside to test the pressure. I get to shoot this sucker over the side. I can barely even hold the nozzle in place. LT Duffy comes in for reinforcement on the hose and I go for it. I sprinkle here, I sprinkle there, hose checks out. Good deal. This was a blast. See what I did there? Later I come to find they had stamped the hose nozzle with my name as a memento. This is such a thoughtful way to remember my time on NOAA Ship Pisces. I shall carry it with me always. Not true, this thing is heavy, but I will certainly cherish it. I have so much respect for our firefighters and first responders (on board and beyond), and even more so today.

Jordan in fire fighting gear (including respirator mask) — Fire fighting gear

Fire station #17, with a folded hose — Fire fighting gear

At this point, I have been out at sea for 12 days. That’s a record for me. My previous PR is one night on a lake in Indiana. I really had no idea what to expect on this trip. I was pretty nervous I would be violently ill and concerned I may not sleep and they wouldn’t have enough coffee to sustain me. None of these were issues, actually far from it, and man am I grateful. No seasickness, I’ve slept like a baby, and there is coffee for days. They even have espresso. Winning. They’ve really spoiled me out here. We have had some really tasty meals, including the fish. No fish goes to waste! I am going to miss being out here at sea. I think I might stick around.

Did You Know?

Wearing gloves, Jordan uses tweezers to hold up an extracted otolith at eye level.

So you now know that otoliths are basically ear bones. What is cool about them is that they grow throughout the life of a fish, leaving traces on the ear bone. Seasonal changes in growth are recorded on the bone and appear as alternating opaque and translucent rings. Under a microscope, scientists count the number of paired opaque and translucent rings, or annuli, to estimate the age of a fish. Just like trees!

July 29, 2017

Brad Rhew: Getting Fishy With It, July 29, 2017

NOAA Teacher at Sea

Brad Rhew

Aboard NOAA Ship Bell M. Shimada

July 23 – August 7, 2017

Mission: Hake Survey

Geographic Area of Cruise: Northwest coast

Date: July 28, 2017

Weather Data from the Bridge

Latitude 4359.5N
Longitude 12412.6 W
Temperatue: 54 degrees
Sunny
No precipitation
Winds at 23.5 knots
Waves at 2-4 feet

Science and Technology Log

We are officially off! It has already been an amazing experience over the last couple of days.

One of the goals of this project is to collect data that will be used to inform the Pacific hake stock assessment. This falls in line with the Pacific Whiting Treaty that the US-Canadian governments enacted to jointly manage the hake stock. NOAA and Department of Fisheries and Oceans-Canada (DFO) jointly survey and provide the hake biomass to the stock assessment scientists. (Refer to the link in my last blog about additional information on this treaty.) Major goals of the survey are to determine the biomass, distribution, and biological composition of Pacific hake using data from an integrated acoustic and trawl survey. Additionally, we are collecting a suite of ecological and physical oceanographic data in order to better understand the California Current Large Marine Ecosystem (CCLME).

There is a very detailed process the scientists go through to collect samples and data on the hake caught and selected for sampling. They want to learn as much as possible about these fish to help with the ongoing research projects.

Here is a quick guide and understanding of how sampling works and what data is collected:

Determine the length and sex of the fish.
1. To determine the length, the fish is placed on a magnetic sensor measuring board. The magnet is placed at the fork of the tail fin; the length is recorded into the data table. (See figure A.)
  Figure A. Determining the length of the fish.
2. To determine the sex, the fish is sliced open on the side. Scientist look to see if ovaries (for females) or testes (for males) are present. They also can determine the maturity of the fish by looking at the development of the reproductive organs. (See figure B.)
  Figure B. Determining the sex of the fish.
Determine the mass.
1. The Hake are placed on a digital scale and then massed. The data also gets entered into the database. (See figure C.)
  Figure C. Massing the fish on a digital scale.
Removing of the otoliths (ear bones).
1. Hake have two otoliths. How this is done is the scientist first cuts a slight incision on top of the fish’s head. (See figure D.)
  Figure D. Making an incision on the fish’s head to remove otoliths.
2. The head is then carefully cracked open to expose the bones. (See figure E.)
3. The bones are removed with forceps and then placed in a vial. The vial is then barcode scanned into the database. The otoliths will then be sent to the lab for testing. Scientists can run test on the otoliths to determine the age of the selected fish. (See figures F and G.)
  
  Figure E. Cracking head open to reveal otolith
  
  Figure F. Removing otolith with forceps
  
  Figure G. Storing otolith in vial
Removing a fin clip.
1. Fin clips are removed from the Hake for DNA sampling to be completed back on shore in the lab. This gives researchers even more information about the selected fish.
2. The fin clip is removed using scissors and forceps. (see figure H.)
  Figure H. Removing a fin clip.
3. The clip is then placed on a numbered sheet. (see figure I.)
  Figure I. Placing the fin clip on a numbered sheet.
4. The number is also entered into the database with all the other information collected on that particular fish.
All the information is collected in one database so it can be assessed by scientists for future research. (see figure J.)
Figure J. All information is stored in a database.

Personal Log

Even though this survey is just beginning this has been such an amazing experience already. I have learned a great deal about oceanography and marine research. I cannot wait to use my experiences back in my classroom to expose my students to careers and opportunities they could be a part of in their future.

Another great aspect of being a Teacher at Sea is the relationships I’m building with other scientists and the crew. It is amazing to hear how everyone became a part of this cruise and how passionate they are about their profession and the world around them.

Did You Know?

This is Leg 3 of 5 of this Summer Hake Survey. Two more legs will be completed this year to collect even more data on the fish population.

Fascinating Catch of the Day!

When we fish for Hake it is very common to collect some other organisms as well. Today’s fun catch was Pyrosomes or Sea Tongues!

These free-floating colonial tunicates are found in the upper part of the open ocean. Pyrosomes rely on the currents to move them around the ocean. They are typically cone shaped and are actually made up of hundreds of organisms known as zooids. The Zooids form a gelatinous tunic that links them together creating the cone shape. They are also bioluminescent and give off a glow in the ocean.

TAS Rhew Blog 2 photo collage — Fun with pyrosomes!

Check it Out!

If you want to learn more about what is happening on the Bell M. Shimada, check out The Main Deck blog for the ship:

https://www.nwfsc.noaa.gov/news/blogs/display_blogentry.cfm?blogid=7

April 17, 2017April 17, 2017

Karen Grady: Sometimes You Find A Little Something Extra, April 16, 2017

NOAA Teacher at Sea

Karen Grady

Aboard NOAA Ship Oregon II

April 5 – April 20, 2017

Mission: Experimental Longline Survey

Geographic Area of Cruise: Gulf of Mexico

Date: April 16, 2017

Weather Data

Latitude 2848.37 N
Longitude 09247.66 W
76 degrees
Sunny
No precipitation
Winds at 11 KTS
Waves at 2-4 FT

Science and Technology Log

Sometimes when a shark or fish is brought on board it has a “hitchhiker’ attached. We caught a blacknose shark that had a common remora, often referred to as a sucker fish, or shark sucker, attached to it. Scientist Kevin Rademacher placed this sharksucker (Echeneis naucrates) on my arm. I couldn’t really feel it but he was stuck there until I peeled him off. It was like peeling a piece of tape off. You can see from the photo how he is designed to attach to host species. Their head is actually a modified dorsal fin that has an oval shaped sucking disk with slat-like structures that open and close to create suction and take a firm hold against the skin of its host animal such as a shark, turtle, whale, or ray. By sliding backward, the remora can increase its suction, or it can release itself by swimming forward. They can be small like the one attached to my arm or they can grow to over two feet in length. The remora can move around on the host, removing parasites while at the same time gaining protection provided by the host. This relationship is often looked at as one of commensalism where both the host and the remora benefit.

The remora’s modified dorsal fin provides suction ability

Remora on TAS Karen Grady’s arm

Remora

Photos of the remora that was attached to a black-nosed shark.

When one hears that this is an experimental long-line survey of sharks and reef fish, all you think of is catching these creatures and collecting data. However, scientists are collecting data about the environment as well. It is very useful to obtain information about the water where they catch large numbers of a species and areas where they may not catch anything. One way they can do this is by using a Conductivity Temperature Depth Profiler (CTD).

The CTD gives scientists a profile of the water column where we just put out our line. The CTD has sensors that collects information on oxygen levels, temperature, water clarity, chlorophyll concentration, and salinity. The CTD is placed in the water and allowed to sit for three minutes to let the oxygen sensors soak and adjust from being on the deck and lowered into the water. The crew lowers it to a depth that is decided based upon the depth to the ocean floor. They like to take it as close to the bottom as possible in order for the information they gather to be as complete as possible. It is allowed to settle, run its scans and then is brought back up to the surface and the sensors are flushed with fresh water. The data is automatically loaded into the database. This information is collected at each station. It takes a joint effort of the deck, science and bridge crews to place the CTD in the water. Walkie talkies are utilized for communicating between all the crew involved in the operation.

Scientist James Sulikowski prepares the CTD for deployment

Scientists can review the data from the CTD immediately

Personal Log

Being at sea with Easter approaching had its moments when I thought of family and friends. We have our Easter traditions and I would be missing them this year. The Easter Bunny (Field Party Chief, Kristin Hannan) decided we needed an early visit this year. I think she was right. The surprise and the treats perked all the science staff up.

TAS Karen Grady 4-16-17 Easter basket

FPC Kristin Hannan asks me often if I have any questions about what they are doing or anything in general. I will be honest… I have gotten so caught up in what we are doing, trying to do my best at whatever job I am working on, and being in awe that I am actually out here that I forget to ask questions about the details. I love the anticipation of what might be on the next hook, I am mesmerized by the sleek lines of the sharks when we have them on board.

TAS Karen Grady 4-16-17 shark liver — Shark liver

When we had one come onboard that was dead due to low oxygen levels in the water where we caught it, we did a dissection on the deck while we waited to put out another line. The animal science nerd in me came to life! I had no idea the liver was the largest organ inside a shark. Think about it …these creatures have no body fat and they store their energy in the liver. Then we looked at the intestines. There is not a lot of room in there so the shark we looked at the intestines are rolled up like you would roll a piece of paper. This gives them maximum absorption area but takes up a limited space.

One thing I think of as we are catching these species is that very few people stop and think about the actual research scientists do to help understand what is needed to maintain healthy populations. It is necessary to do these surveys, catch the species, tag some, draw blood, take fin clips, keep whole specimens, and dissect some. On our cruise we were lucky enough to ultrasound a few pregnant sharks and see the pups inside.

TAS Karen Grady 4-16-17 shark ultrasound — Baby sharks visible on ultrasound

Now stop and think about all those things I just listed that we do at times. When a hook comes up and there is a fish or shark on it is handed off to one of the science crew. It is noted in the computer that there was a something caught. The science crew member will take measurements and weight of the fish or shark. If it is a shark, the sex will be noted and some species may be tagged, have a fin clip taken and blood drawn. While all of these activities are taking place, the next hooks keep being brought up. The deck can get pretty crazy if there are several hooks in a row with something on them. The data collector has to keep tag numbers, species, measurements, samples and weights all written in the correct spot while having two or three people calling them out for different fish and or sharks. I had experience working cattle which would mean filling syringes, writing down tag numbers, filling taggers, etc. But this is even crazier than that could get at times. And everything stops if someone calls “hardhats” because that means we have one big enough for the cradle. Working back writing down data or taking measurements you can’t see what is on the next line so you sneak up for a peak when they say it’s a big one then you get out of the way. One of the best experiences so far was almost getting a big tiger shark in the cradle. I was lucky enough to get a video of her, so stay tuned! Unfortunately, when the big shark brushed against the cradle she snapped the line and was gone with a huge spray of water.

This second leg of the experimental long-line survey is winding down. There have been long days but they are filled with laughter, giggles, anticipation, excitement, teachable moments (I can finally get the circle hooks out by myself…sometimes) , and the dreaded words “snapper.” I mean nothing against the Red Snapper, they are a bright colorful and tasty fish, but when you are hoping for a shark to be on the hook…. let’s just say the sets where we get 12 snapper and two sharks are not our favorites.

Photos: “Shark!” or “Fish on!” means a busy deck.

TAS Karen Grady 4-16-17 hammerhead cradle — Scalloped hammerhead shark

When the guys at the rail grab the hard hats it means it is time for the cradle and we get to see things like this gorgeous scalloped hammerhead. Things move very quickly when one is in the cradle. Safety for those on deck comes first and everyone is focused on getting measurements, fin clip and a tag on the shark and getting it safely back in the water as quickly as possible.

TAS Karen Grady 4-16-17 baby tiger shark — Baby tiger shark

Baby tiger shark in the cradle. They warned me that they were cute and they were so right. Yes, a shark can be “cute” when your referring to baby tiger sharks and baby hammerheads!

Did You Know

Sharks store energy in their liver. It is the largest organ in their body. The heart on the other hand is extremely small in comparison to the size of the shark.

TAS Karen Grady 4-16-17 hammerhead dissection — Dissected scalloped hammerhead with liver visible

Look at the liver of this scalloped hammerhead. It is amazing how big it is in relation to the body of the shark. This is just one way these amazing creatures are designed to be efficient and survive in their underwater world.

Sharks have a nictitating membrane that they can close over their eye for protection. When a shark is brought on deck you can touch near the eye and the membrane will automatically move to close.

TAS Karen Grady 4-16-17 nictitating membrane — Nictitating membrane partially closed on the eye of a scalloped hammerhead