Tag: calcofi

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 7, 2007

Weather from the Bridge 
Visibility: clear
Wind direction: NW
Sea wave height: 5-8 ft.
Sea temperature: 12.79 C
Air temperature: 14.7 C
Swell wave: 5-8 ft.
Sea Level pressure: 1016.
Cloud cover: partly cloudy

Science and Technology Log 

Up at 06:30. Breakfast and watched with mammal observer on flying bridge.  Saw a few albatross. Very rough water, windy, cold.

Cast 21, 22 and 23 taken by other teams.

Cast 24 @ 08:55 Station 67-75 Latitude  35.5749N Longitude 123.504491 W Cast depth 1000 meters CTD cylinders tripped at 1000, 2000, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters Very windy. Data for cast is Table 12 at the end of the report

Cast 25 @ 11:35 Station NPS 5 Latitude  36.026137 N  Longitude 123.400087 W   Cast depth 1000 meters CTD cylinders tripped at 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50, 0 meters Nutrient samples only taken at this cast.  Data for cast is Table 13 at the end of the report.  Very windy (23 knots)

Spent time again on the flying bridge with mammal observer.  She said on the Beaufort Scale winds above 4 one doesn’t expect to see wildlife out in the ocean. Beaufort scale today is “5”.

Casts 26, 27, and 28 ( wind 26 knots) processed by other teams.

Cast 29 @ 21:27 Station NPS 3 Latitude 36.22583N Longitude 122.57275 W Cast depth 1000 meters CTD cylinders tripped at 1000,900, 800, 700, 600, 500, 400, 300, 200, 100, 50, 0 meters Nutrients samples only collected at this cast. Very windy (wind 22 knots) and water is rough. Data for cast is Table 14 at the end of the report. Worked on chlorophyll analysis.

Took photos of some of the net tow specimen jars to show the extreme of near shore and out at sea differences in material.  Specimens observed today–some shrimp, a few jellyfish, a squid, pteropods, heteropods.  There is not the large amount of krill as observed in the net tow collections closer to shore.

Cast 30 @ 23:37 Station 67-60  Latitude  36.275608 N  Longitude 122.466380 W Cast depth 1000 meters CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters Very windy (23 knots) Samples processed and stored Data for cast is Table 15 at the end of the report.

Bed 01:00 June 8th

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 6, 2007

Weather from the Bridge 
Visibility: clear
Wind direction: 291
Wind speed: 16 knots
Sea wave height: 2-3 ft.
Swell wave: 5-7ft.
Sea temperature: 14.671 C
Air temperature: 16.1 C
Sea level pressure: 1021
Cloud cover: 25% scattered cumulus

Science and Technology Log 

Up at 07:00 Discussion continued on how to do deep casts with CTD and avoid kink in wire: lower it slower or put on more weight or etc.  Some staff short on sleep after working with CTD repair last night. I do fine on six hours a night but I feel it when it’s five.  I will try for a nap today.

Cast 13 and 14 were done with other staff and went without problems.  They will try a deep cast again today.

Cast 15 08:00 Station 65-90 Latitude 35.03387N  Longitude -127.45604 W at depth to 1000 m; CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters In the wet lab work the funnel for sample #8 was not locked tightly and the apparatus leaked. I put on a new filter and took another seawater sample for #8.  Samples processes and stored. Data for cast is Table 7 at the end of the report.

The two 4′ by 6′ incubators on deck contain the C14 spiked samples placed in a continually flowing seawater bath for twenty-four hours.  Samples are placed in metal tubes with various numbers of holes in the tubes.  The various tubes are designed so that the samples are exposed to 50%, 30%, 15%, 5% and 1%.  One set of samples is not in tubes, but in full sunlight. Then they are evaluated for the rate the phytoplankton incorporate the Carbon 14 as described in Day 3.

Began chlorophyll analysis on the filtered specimens from the range of depths at each station that have been in the freezer more than twenty-four hours.

Marguerite went over the procedure using the flurometer to process the sample. It must be turned on at least one hour before running the tests and the chlorophyll samples #1-12 plus 1 and 5 micron samples must be at room temperature in the dark for at least one hour before beginning. She calibrated the flurometer with acetone.  We rinse the cuvette three times with a couple of milliliters of sample, and then add the remainder to the cuvette.  It will be about 2/3 full or more.  Wipe the cuvette well with a lab wipe to remove any oil on glass from your hand/fingers, place sample gently into flurometer.  The first reading should be taken after it has stabilized, usually 15-20 seconds.  Then two drops of 5% hydrochloric acid are added to degrade the chlorophyll pigment.  A second reading is taken to measure the remaining pigment. These are recorded on a “Bottle Sample Data Sheet”, an example of which is included as Table 8 at the end of the report.  After measurements are recorded, the sample is thrown out in a collection container and the vials disposed of in a waste container.

The cuvette is rinsed three times with acetone and then begin processing the next sample. Again it really helped to have seen this procedure demonstrated on the DVD that was sent to me ahead of the trip.  I was much better prepared.  It was important for the research done as well because if one made a mistake in the sample procedure, there was no sample in reserve to be able to run the test again. I did samples for a couple of hours in the afternoon and a couple more in the evening when I was scheduled for working but waiting for a cast to come up.

Cast 16 and 17 were processed by other team.

Cast 18 @ 15:35 Station 67-90 Latitude 35.4670N  Longitude -124.9409 W Cast depth 4380 went very well. Processed by Erich and Charlotte. Cylinders tripped at 4380 bottom, 4000, 3500, 3000, 2500, 2000, 1500, 1000, 750, 500, 250, 0 meters; Data for cast as Table 9 at the end of the report.

I observed a couple of bongo net tows today. Live net tows are collecting zooplankton and other seawater specimens from the first 200 meters of depth.  The bongo nets have two .8-meter diameter rings with a mesh net and a polycarbonate tube at the end.  The nets were deployed using the ship’s starboard winch equipped with at least 300 meters of wire. The ship maintains a vertical wire angle during the tow of approximately 45 degrees. Kit Clark, the oceanographer in charge of net tows said it was important that the winch be able to maintain a slow, constant retrieval speed.  When nets are retrieved, they are hosed down to wash specimen sticking to the mesh down into the polycarbonate tube. The specimens are transferred to jars and fixed with formalin. There were a lot of krill and one viper eel in the specimens I observed this morning.

Wildlife observer saw three albatross today.  Cast 19 @ 21:14 Station-NPS-8 Latitude 35.325665 N  Longitude 124.438304 W  Cast depth 1000 meters; Cylinders tripped at 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50, 0 meters; Nutrient samples only taken for this cast; Data for cast is Table 10 at the end of the report.

Cast 20 @ 11:29 Station 67-85 Latitude 35.6249 N Longitude -124.5544W  Cast depth 1000 meters; CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters; Bottle # 2 leaked, was empty, so no sample collected. Always check that funnels are locked tight before I begin. Samples processed and stored; Data for cast is Table 11 at the end of the report.

Long discussion of the structure and movement of ocean currents.  Dr. Collins is a brilliant scientist, such depth in oceanography.  He uses vocabulary during his explanations that need explanation in themselves. The Great Lakes and fresh water bodies are a lot simpler.

Discussed with Dr. Collins the military value of the studies we are doing.  He said the military does sea floor mapping, looks for mines and things on the sea floor. He explained that there are levels of optimum transmission of sound, channels for submarines.  Determining these best channels relates to the salinity and temperature

Bed @ 01:00 June 7th

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 5, 2007

Weather from the Bridge 
Visibility: Clear
Wind direction 275.64
Wind speed: 13 knots
Air temperature: 16.1 C
Sea wave Height: 1-2 feet
Seawater temperature: 13.98 C
Swell wave: 4-6 feet
Sea level pressure: 1017.4
Cloud cover: 50%, stratus

Science and Technology Log 

Up at 06:00 and went to flying bridge to observe with Kathryn.  Not much wildlife to see other than a few sea gulls. Color of water so blue.  Temperature is cool early in the morning. Breakfast good fruit, lots of starches, sausages.  A time to talk to crewmembers about the different trips of MCARTHUR II from Alaska to Peru.  Jim spoke in detail @ working as a fisherman in Alaska, ice in his moustache, not needing very heavy clothes because you worked so hard you got hot.  He said it was 06:00 until 22:00 in summer time.  He spoke about fishing limit rules, coordinating with Japanese fishing boats, and also how the catch numbers have fallen since ten or fifteen years ago.

Cast 6 and 7 were early in the morning with other cruise staff. All proceeded as expected.

Cast 8 @ 08:18 Station 60-75 Latitude 37.067N Longitude 124.4145 W Cast depth to 1000m; CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters Data for cast is Table 5 at end of report. Cast information time is always written in Greenwich time; I subtract seven hours to show our time on ship for the station work.  The Cast information listing for latitude and longitude is close but not exact to Table 1 for the CalCOFI stations. In the 1000 meter depth casts it takes about forty minutes for the CTD to go down to depth and come up again, stopping at the different levels for the specific rosette to open.

I learned more information on the testing of samples from Marguerite Blum, MBARI oceanographer: The nutrient samples contain nitrates and nitrites as well as silicates, phosphates.  The nitrates and nitrites area examined at Moss Landing lab with an auto flow analyzer, which translates sample into voltage and indicates the amount of the nutrient in the sample.

QP (quantitative phytoplankton) will show up to fifteen general types of phytoplankton in a sample.  This is an expensive test to run.  The flow cytometry test divides the sample into four groups: bacteria, prokaryotes, eukarotyes, and zooplankton.  It will determine a general number of how many of each are present in the sample.

The Carbon 14 test shows the amount of carbon uptake by the phytoplankton.  C14 of specimen fluoresces and radioactive emission level counted on a scintillation counter. The chlorophyll analysis of the green chlorophyll is run on the flurometer.  Samples that have been in the freezer 24 hours we will process in the dry lab while on this cruise.  On this cast I also handled the A* filter, the HPLC filter and the POC filter, placing them in their red, blue, and green cryovials respectively, and then put in the liquid nitrogen container. The analysis of HPLC, POC, FCM and N15 samples are not done at Moss Landing but are sent out to other labs for processing.

Cast 9 @ 11:45 Station 60-80 Latitude 36.5677N  Longitude 125.0327 W Cast depth to 1000m; CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters; Data for cast is Table 6 at end of report.

There have been bongo net tows at our stations, but often when I am working or sleeping. I have seen some of the specimens caught which are in jars with formalin.  I hope to see a net tow start to finish soon.

I have watched with Kathryn, the Mammal observer, during different periods today and have not seen any wildlife. She saw seven dolphins earlier in the day.  I asked her about the tagging of sea life and she talked about the guidelines. She said the organization had to apply for a permit to the National Marine Fisheries.  This may take up to a year to obtain. A boat doing tagging must display a special flag with a research number on it. The permit will specify the number of “takes” (getting close to or affecting the animal such as a whale or turtle).  She said a lot of information was available on line at TOPP (Tagging of Pacific Pelagics). When it’s on the surface, the signal from the tagged animal will beam up to satellite and transmit its location. How long it will function depends on the battery life, and of course a small animal can only carry a small battery.  The scientist will set the frequency of the beam according to the frequency of the animal at the surface. A sea lion surfaces every fifteen minutes so its battery will last three weeks.  A turtle will surface every second day so its battery will last six months to a year or more. Scientists want to recapture radio equipment so watch closely at the animal’s location.  The equipment will give off a signal for at least a week after it falls off.  Researchers put gummy worms under the suction cups on whales and know it will take about a week for that sugar to be dissolved, and then the apparatus will fall off.  Tic Tacs with suction cups also work.. The equipment is numbered for location and will be returned if found by any ships, any countries at sea. She said a problem that can occur is that is that the sensor on the collar could get algae grown over it so it stays off.

Cast 10 and 11 were with other cruise staff.

Cast 12 was started @ 22:45 and was to be a deep cast, 4500m.  When it reached @1100m transmission of data stopped.  The CTD was brought back to the surface and worked on by staff about three hours. A kink had developed in the wire.  That section was cut out and all connections redone.

Data retrieved gives information for the 1100 m at this location for beam transmission, salinity, temperature, and fluorescence all taken by the computer monitoring system in the dry lab. Bottle samples were not taken.

To bed @ 02:00 June 6th I am greatly stewing about the CTD problems with all it means to the research, to the cruise,  and the expense of it all.

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 3, 2007

Weather DAY 1: San Francisco, Pier 30/32 
Visibility: 10 nautical miles
Wind direction: 270 NW
Wind Speed: 8 knots
Sea wave height in harbor: 1′
Seawater temperature: 15.129 C.
Sea level pressure: 1016.4
Air temperature: 15.2
Cloud cover: 1/4 cumulus

Science and Technology Log 

The day began @ 07:30 picking up equipment at Moss Landing and riding up to San Francisco in van with other MCARTHUR II cruise members: Chief Scientist Tim Pennington, Biological Oceanographers-Marguerite Blum, Kit Clark, Erich Rienecker, Troy Benbow, Charlotte Hill; Physical Oceanographer, Dr. Curt Collins; CTD technician, Doug Conlin. At Pier 30/32, Marine Mammal Biologist, Katherine Whitaker, joined us and the other Teacher at Sea participant, Turtle Haste.

Tim Pennington coordinated the staging operation with the (FOO) Field Operation Officer Lt. Amanda Middlemiss.  The large equipment for the cruise was at the pier on a flat bed truck and was loaded by crane on the ship’s deck with the assistance of the ship’s crew. All scientists were involved in unpacking the gear and setting up the wet lab and dry lab for the Time Series study work.  As these labs have been physically updated since the last MBARI cruise on MCARTHUR II, set up in these labs required some modifications. All staff commented on the benefits and advantages of the lab improvements.

I reviewed material I researched on line prior to cruise about the Monterey Bay Aquarium Research Institute (MBARI) Time Series program.  The focus is on the relations between oceanic carbon and nitrogen cycles and climate variability with emphasis on measuring the primary phytoplankton production.  The research involves both observational and experimental studies with shipboard measurements of physical, chemical and biological parameters during cruises in Monterey Bay (since 1989) and offshore into the California Current (since 1997) at different seasons of the year.  The data collected over this time span is being used to construct synthetic views of the oceanographic system dynamics of the California Current. The work has documented seasonal cycles, El Ninos and La Ninas and longer decade-scale cycles (e.g., Pacific Decadal Oscillation).  The overall goal is to learn as much as possible about the earth’s climate and ocean systems, and therefore it is important to understand these cycles. Beyond construction of views of the California Current cycles and understanding the causation of them, will scientists determine that the directions show potential effects of global warming?

As stated in the summary of the MBARI Time Series Program report 2007: “Is this a local-or remotely-driven effect?  We are uncertain. Is it important? You bet.  Why? Because we area certain that (1) conclusions about global climate change begin with local observations, and (2) unusual conditions are often highly informative.”

Chief Scientist (CS) Tim Pennington went over the wet lab organization with the three of us new to working there, defining the different sample bottles and chemicals used in collecting and processing the sea water samples.  He showed us which type of samples were stored in the freezer or in the liquid nitrogen, and which were placed in the seawater bath on the back deck. We signed up for our individual research tasks, my assignment is seawater sample collection from the rosette bottles of the CTD and processing in the wet lab. When filtered samples are ready, to process with the flurometer for chlorophyll level. My shift is 08:00 – 12:00 and 20:00-24:00.  I work with CS Tim. Then we are free to study/work in other areas as you would like or as you are needed.  We put duct tape ridge along front edge of wet lab tables to help stop materials from sliding off counter if ship is rolling.

At 16:00 we moved our personal belongings to our assigned quarters and then were free to explore the set-up of the MCARTHUR II. Important to note were the areas where one must wear a hard hat and a PFD. No open toed footwear outside your quarters. Pay attention to stay far away from winches when they are being used.

FOO Lt. Middlemiss requested that we review the safety instructions packet found in our quarters and that we should be ready for the safety drill to take place the next day.

Bed at 00:30 June 4th.