Beth Lancaster, April 7, 2008

NOAA Teacher at Sea
Beth Lancaster
Onboard NOAA Ship McArthur II
April 6 – 14, 2008

Mission: Examine the spatial and temporal relationships between zooplankton, top predators, and oceanographic processes
Geographical area of cruise: Cordell Bank Nat’l Marine Sanctuary & Farallones Escarpment, CA
Date: April 7, 2008

Beth Lancaster (right) preserves a plankton sample collected using a hoop net.
NOAA Teacher at Sea Beth Lancaster bottles a surface water sample that will be tested for the presence of nutrients.

Science and Technology Log 

Today was the first full daytime operations.  We began shortly after 7:00 a.m., and covered a 90 kilometer transect throughout the course of the day ending at 6:00 p.m.  At each sampling point along the transect a series of measurements and observations were made to look at relationships between the physical ocean environment, and abundance of living organisms that are observed and collected to gain a better understanding of the physical and biological features of the area, and how they interact. The daytime crew was divided into two groups: the marine mammal and bird observers, and a second group that was responsible for collecting water and plankton samples as well as other various physical measurements of the water.  I worked with the second group, and will share what sampling I assisted with.

At each sampling point we used the CTD, which is a piece of equipment that has several probes on it, to collect a vertical sample of the water column.  When the CTD is deployed into the water it is sent down 200 meters below the surface and collects water conductivity (used to calculate salinity), temperature, depth, and turbidity. There is also a fluorometer attached to the CTD that measures the fluorescence of chlorophyll-a, which approximates the abundance of phytoplankton.  The CTD collects all this data, and can then be downloaded onto a computer.  Surface water samples were also collected at each sampling point, and will be tested for the presence of nutrients which would also have a direct impact on the abundance of organisms in the area.

Beth Lancaster (right) preserves a plankton sample collected using a hoop net.
Beth Lancaster (right) preserves a plankton
sample collected using a hoop net.

To gather information on the living organisms present at each site, a hoop net was used to collect samples of plankton.  The net was sent down approximately 50 meters, and collected all of the tiny living organisms (zooplankton) on a screen as the net was pulled through the water column. When the hoop net was brought back onboard, the cod end of the net (where the sample is collected) was transferred to a sample bottle, and preserved for further investigations in the laboratory. In addition to the living organisms collected in the hoop net, marine mammal and bird observations are being made from the flying bridge of the ship. That would be the highest point on the boat, and not the location for people who are afraid of heights. Due to rough sea conditions (10-12 foot swells), sightings were few and far between today.  Springtime within Cordell Bank National Marine Sanctuary is a time where strong winds cause upwelling of deeper waters towards the surface near the coast.  This upwelled water is colder and has higher nutrient concentrations.

Sample of krill caught in the daytime with a hoop net.
Sample of krill caught in the daytime with a hoop net.

This influx in nutrients means the ecosystem becomes very productive. Given this high influx of nutrients, prey items for birds and mammals are readily available. The food of choice for a lot of these organisms is krill (a shrimplike zooplankton.)  We did collect some krill in the hoop net during the day, but the abundance of krill in shallower water is much greater in the evening, when krill migrate from deep depths towards the surface.  The night crew is collecting krill using a tucker trawl, which has three separate nets that are opened and closed at different depths. Krill play a vital role in the ecosystem scientists are currently studying. They provide nourishment for resident and migratory birds as well as marine mammals.  There is sufficient nutrient availability for primary producers which are then food for primary consumers such as krill, and therefore food availability for secondary consumers such as fish and tertiary consumers such as whales and dolphins.

Black-footed Albatross
Black-footed Albatross

Throughout the week the same measurements will be taken at different sights along the continental shelf and continental slope in the region of Cordell Bank National Marine Sanctuary and the Farallones Escarpment (within Gulf of the Farallones National Marine Sanctuary). This information will allow scientists to better understand the dynamic relationship between zooplankton, top predators, and oceanographic processes.  Data gathered will also be used in conservation planning of the marine sanctuaries.

Some Animal Sightings 
Black-footed Albatross, Ancient Murrelet, Northern Fulmar, Laysan Albatross, and Pacific White-sided Dolphin.

Elsa Stuber, June 9, 2007

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 9, 2007

Science and Technology Log 

Up at 06:00 and arrived in San Francisco @ 08:00  Unloaded equipment, mostly by winch onto truck; cleaned quarters and wet and dry labs.

In preparing my report I have included the data from the casts I worked on. As they were a dollar each to reproduce at Kinkos, I decided to only include those four-colored graphs for beam transmission, depth, salinity, and temperature that illustrate different types of locations, near shore or at sea of varying depths. These graphs certainly show the increase in salinity with depth and the falling of temperature with depth.  They show the inverse relationship between the beam transmission and fluorescence. Compare Table 3, Table 6, Table 7, and Table 9 that are from very different depths. There is a lot of information here for my students to analyze.

This has been a stimulating learning experience.  My students like most high school students are not near the ocean, maybe have never been to an ocean.  I think sharing this first hand experience along with the pictures and doing demonstrations of our work and showing the data collected will open a perspective in their minds about the ocean.  They will realize from the chlorophyll analysis and fluorescence the narrow level of the bottom of the food chain. This will bring home the concern for the protection of the health of the biotic life in our oceans.  I intend to do a mock set up of our lab procedures and have them brainstorm how experimental error could be introduced.  This should reinforce the importance of careful procedure in any scientific work.

The scientific staff on this cruise has been exceptional in willing to share their knowledge and, even when we were all tired, to answer my many, many questions about each individuals work in oceanography.  I have found them to be conscientious scientists very interested, stimulated in their work. Some have given me pictures.  Several have done extended work in Antarctica, which was particularly interesting to me. As well, the MBARI staff was very supportive as the other Teacher At Sea and I were learning procedures for our work. It felt good to see how cooperative, flexible they all were with one another no matter how long the days.  I wish we could have been able to go to the MBARI lab this coming week to see how the work continues there.  I intend to go on line and read more about their research and research findings. This will be something to investigate and follow with my students and extending their understanding of the oceans and the oceanographers’ work.

Elsa Stuber, June 8, 2007

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 8, 2007

Weather from the Bridge 
Visibility: clear
Wind direction 282 NW
Wind speed: 18.9 knots
Sea wave height: 3-5 feet
Sea temperature: 10.5 C
Air temperature: 13.5 C
Sea level pressure: 1013.36
Cloud cover: 100 % status clouds

Science and Technology Log 

Wind woke me up at 06:00, boat rolling.  Early morning 03:00—05:00 winds were 30 knots. Casts 31, 32, and 33 processed by other teams.

Cast 34 @ 09:24 Station H3  Latitude 36.44117 N  Longitude 122.01108 W Cast depth 1000 meters CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters Samples processed and stored.  Data for cast is Table 16 at the end of the report.  Worked on chlorophyll analysis with flurometer.

Cast 35 @ 11:47 Station C1  Latitude 36.478487N  Longitude 121.508392 W Cast depth 225 meters CTD cylinders tripped at 225, 200,. 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters Samples processed and stored.  Data for cast is Table 17 at the end of the report. I worked on chlorophyll analysis off and on throughout the day.

The HyperPro instrument to measure light up to 40 meters depth in the water has been tested at mid-day each day.  One tube is pointed down and opposite tube is pointed up sensing light levels. A third tube is strapped to the railing registering light levels at all times.  Seechi was used during the daylight hours as well. MBARI staff gave us some Styrofoam cups, two sizes, to decorate as we wanted using different permanent colored markers.  We put all of them in a mesh laundry bag and attached it to a 1000-meter depth cast.  When they came back up they had shrunk to 1/6th of the original size. It demonstrates the amount of air in the Styrofoam, which should be a good illustration for my students.

Wildlife observations: humpback whales, dolphins, sea gulls, cormorants, sooty shearwaters, and albatross. Kathryn said the sooty shearwater cannot take off from the ground very well. This bird will climb up the trunk of a tree a ways and take off from there. They will wear the bark down going up a path on the tree.  She hoped we would see a Yaeger bird, a bird that chases other birds that have been feeding, making them drop their food. That’s how the Yaeger feeds. It is very aggressive she said in pursuing other birds.

Moved to an area in Monterey Bay where whales had been sighted.  Saw five at a distance of half a mile, sometimes a fin, but mostly the whale’s spout from the blowhole.

Packing up equipment so ready to unload early tomorrow in San Francisco.

Each day the plan of the day is posted by the FOO.  I include an example at the end of the report.

We did extra stations as we are ahead of schedule.   Cast 36 @23:58 nutrients only. Final station done by Troy, nutrients only at 03:00 June 9, 2007

Bed at 01:00 June 9th

Elsa Stuber, June 7, 2007

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 7, 2007

Weather from the Bridge 
Visibility: clear
Wind direction: NW
Sea wave height: 5-8 ft.
Sea temperature: 12.79 C
Air temperature: 14.7 C
Swell wave: 5-8 ft.
Sea Level pressure: 1016.
Cloud cover: partly cloudy

Science and Technology Log 

Up at 06:30. Breakfast and watched with mammal observer on flying bridge.  Saw a few albatross. Very rough water, windy, cold.

Cast 21, 22 and 23 taken by other teams.

Cast 24 @ 08:55 Station 67-75 Latitude  35.5749N Longitude 123.504491 W Cast depth 1000 meters CTD cylinders tripped at 1000, 2000, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters Very windy. Data for cast is Table 12 at the end of the report

Cast 25 @ 11:35 Station NPS 5 Latitude  36.026137 N  Longitude 123.400087 W   Cast depth 1000 meters CTD cylinders tripped at 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50, 0 meters Nutrient samples only taken at this cast.  Data for cast is Table 13 at the end of the report.  Very windy (23 knots)

Spent time again on the flying bridge with mammal observer.  She said on the Beaufort Scale winds above 4 one doesn’t expect to see wildlife out in the ocean. Beaufort scale today is “5”.

Casts 26, 27, and 28 ( wind 26 knots) processed by other teams.

Cast 29 @ 21:27 Station NPS 3 Latitude 36.22583N Longitude 122.57275 W Cast depth 1000 meters CTD cylinders tripped at 1000,900, 800, 700, 600, 500, 400, 300, 200, 100, 50, 0 meters Nutrients samples only collected at this cast. Very windy (wind 22 knots) and water is rough. Data for cast is Table 14 at the end of the report. Worked on chlorophyll analysis.

Took photos of some of the net tow specimen jars to show the extreme of near shore and out at sea differences in material.  Specimens observed today–some shrimp, a few jellyfish, a squid, pteropods, heteropods.  There is not the large amount of krill as observed in the net tow collections closer to shore.

Cast 30 @ 23:37 Station 67-60  Latitude  36.275608 N  Longitude 122.466380 W Cast depth 1000 meters CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters Very windy (23 knots) Samples processed and stored Data for cast is Table 15 at the end of the report.

Bed 01:00 June 8th

Elsa Stuber, June 6, 2007

NOAA Teacher at Sea
Elsa Stuber
Onboard NOAA Ship McArthur II
June 4 – 9, 2007

Mission: Collecting Time Series of physical, chemical and biological data to document spatial and temporal pattern in the California Current System
Geographical Area: U.S. West Coast
Date: June 6, 2007

Weather from the Bridge 
Visibility: clear
Wind direction: 291
Wind speed: 16 knots
Sea wave height: 2-3 ft.
Swell wave: 5-7ft.
Sea temperature: 14.671 C
Air temperature: 16.1 C
Sea level pressure: 1021
Cloud cover: 25% scattered cumulus

Science and Technology Log 

Up at 07:00 Discussion continued on how to do deep casts with CTD and avoid kink in wire: lower it slower or put on more weight or etc.  Some staff short on sleep after working with CTD repair last night. I do fine on six hours a night but I feel it when it’s five.  I will try for a nap today.

Cast 13 and 14 were done with other staff and went without problems.  They will try a deep cast again today.

Cast 15 08:00 Station 65-90 Latitude 35.03387N  Longitude -127.45604 W at depth to 1000 m; CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters In the wet lab work the funnel for sample #8 was not locked tightly and the apparatus leaked. I put on a new filter and took another seawater sample for #8.  Samples processes and stored. Data for cast is Table 7 at the end of the report.

The two 4′ by 6′ incubators on deck contain the C14 spiked samples placed in a continually flowing seawater bath for twenty-four hours.  Samples are placed in metal tubes with various numbers of holes in the tubes.  The various tubes are designed so that the samples are exposed to 50%, 30%, 15%, 5% and 1%.  One set of samples is not in tubes, but in full sunlight. Then they are evaluated for the rate the phytoplankton incorporate the Carbon 14 as described in Day 3.

Began chlorophyll analysis on the filtered specimens from the range of depths at each station that have been in the freezer more than twenty-four hours.

Marguerite went over the procedure using the flurometer to process the sample. It must be turned on at least one hour before running the tests and the chlorophyll samples #1-12 plus 1 and 5 micron samples must be at room temperature in the dark for at least one hour before beginning. She calibrated the flurometer with acetone.  We rinse the cuvette three times with a couple of milliliters of sample, and then add the remainder to the cuvette.  It will be about 2/3 full or more.  Wipe the cuvette well with a lab wipe to remove any oil on glass from your hand/fingers, place sample gently into flurometer.  The first reading should be taken after it has stabilized, usually 15-20 seconds.  Then two drops of 5% hydrochloric acid are added to degrade the chlorophyll pigment.  A second reading is taken to measure the remaining pigment. These are recorded on a “Bottle Sample Data Sheet”, an example of which is included as Table 8 at the end of the report.  After measurements are recorded, the sample is thrown out in a collection container and the vials disposed of in a waste container.

The cuvette is rinsed three times with acetone and then begin processing the next sample. Again it really helped to have seen this procedure demonstrated on the DVD that was sent to me ahead of the trip.  I was much better prepared.  It was important for the research done as well because if one made a mistake in the sample procedure, there was no sample in reserve to be able to run the test again. I did samples for a couple of hours in the afternoon and a couple more in the evening when I was scheduled for working but waiting for a cast to come up.

Cast 16 and 17 were processed by other team.

Cast 18 @ 15:35 Station 67-90 Latitude 35.4670N  Longitude -124.9409 W Cast depth 4380 went very well. Processed by Erich and Charlotte. Cylinders tripped at 4380 bottom, 4000, 3500, 3000, 2500, 2000, 1500, 1000, 750, 500, 250, 0 meters; Data for cast as Table 9 at the end of the report.

I observed a couple of bongo net tows today. Live net tows are collecting zooplankton and other seawater specimens from the first 200 meters of depth.  The bongo nets have two .8-meter diameter rings with a mesh net and a polycarbonate tube at the end.  The nets were deployed using the ship’s starboard winch equipped with at least 300 meters of wire. The ship maintains a vertical wire angle during the tow of approximately 45 degrees. Kit Clark, the oceanographer in charge of net tows said it was important that the winch be able to maintain a slow, constant retrieval speed.  When nets are retrieved, they are hosed down to wash specimen sticking to the mesh down into the polycarbonate tube. The specimens are transferred to jars and fixed with formalin. There were a lot of krill and one viper eel in the specimens I observed this morning.

Wildlife observer saw three albatross today.  Cast 19 @ 21:14 Station-NPS-8 Latitude 35.325665 N  Longitude 124.438304 W  Cast depth 1000 meters; Cylinders tripped at 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50, 0 meters; Nutrient samples only taken for this cast; Data for cast is Table 10 at the end of the report.

Cast 20 @ 11:29 Station 67-85 Latitude 35.6249 N Longitude -124.5544W  Cast depth 1000 meters; CTD cylinders tripped at 1000, 200, 150, 100, 80, 60, 40, 30, 20, 10, 5, 0 meters; Bottle # 2 leaked, was empty, so no sample collected. Always check that funnels are locked tight before I begin. Samples processed and stored; Data for cast is Table 11 at the end of the report.

Long discussion of the structure and movement of ocean currents.  Dr. Collins is a brilliant scientist, such depth in oceanography.  He uses vocabulary during his explanations that need explanation in themselves. The Great Lakes and fresh water bodies are a lot simpler.

Discussed with Dr. Collins the military value of the studies we are doing.  He said the military does sea floor mapping, looks for mines and things on the sea floor. He explained that there are levels of optimum transmission of sound, channels for submarines.  Determining these best channels relates to the salinity and temperature

Bed @ 01:00 June 7th